GIF Antibody (1D9)
Novus Biologicals, part of Bio-Techne | Catalog # H00002694-M03
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 1D9
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
GIF (NP_005133.2, 318 a.a. ~ 417 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. INNQLRGVELLFNETINVSVKSGSVLLVVLEEAQRKNPMFKFETTMTSWGLVVSSINNIAENVNHKTYWQFLSGVTPLNEGVADYIPFNHEHITANFTQY
Specificity
GIF - gastric intrinsic factor (vitamin B synthesis) (1D9)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for GIF Antibody (1D9)
Western Blot: GIF Antibody (1D9) [H00002694-M03]
Western Blot: GIF Antibody (1D9) [H00002694-M03] - Analysis of GIF transfected lysate using anti-GIF monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with GIF rabbit polyclonal antibody.Immunocytochemistry/ Immunofluorescence: GIF Antibody (1D9) [H00002694-M03]
Immunocytochemistry/Immunofluorescence: GIF Antibody (1D9) [H00002694-M03] - Analysis of monoclonal antibody to GIF on HeLa cell. Antibody concentration 10 ug/ml
Sandwich ELISA: GIF Antibody (1D9) [H00002694-M03] - Detection limit for recombinant GST tagged GIF is approximately 0.3ng/ml as a capture antibody.
Applications for GIF Antibody (1D9)
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Sandwich ELISA
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
It has been used for ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: GIF
Long Name
Gastric Intrinsic Factor
Alternate Names
IF, IFMH, INF, TCN3
Entrez Gene IDs
2694 (Human)
Gene Symbol
CBLIF
UniProt
Additional GIF Products
Product Documents for GIF Antibody (1D9)
Product Specific Notices for GIF Antibody (1D9)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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