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GILT/IFI30 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83366

Novus Biologicals, part of Bio-Techne
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NBP1-83366
NBP1-83366-25ul

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LVPYGNAQEQNVSGRWEFKCQHGEEECKFNKVEACVLDELDMELAFLTIVCMEEFEDMERSLPLCLQLYAPGLSPDTIMECAMGDRGMQLMHANAQRTDALQPPHEYVPWVTVNGKPLEDQTQLLTLVCQLYQGKK

Reactivity Notes

Mouse reactivity reported from a verified customer review.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GILT/IFI30 Antibody

GILT/IFI30 Antibody

Immunohistochemistry-Paraffin-GILT/IFI30 Antibody-NBP1-83366-

Immunohistochemistry-Paraffin-GILT/IFI30 Antibody-NBP1-83366-Analysis in human lung and skeletal muscle tissues using NBP1-83366 antibody. Corresponding IFI30 RNA-seq data are presented for the same tissues.
GILT/IFI30 Antibody

Immunohistochemistry-paraffin-GILT/IFI30 Antibody-NBP1-83366-

Immunohistochemistry-paraffin-GILT/IFI30 Antibody-NBP1-83366-Staining of human tonsil shows strong cytoplasmic positivity in a subset of non-germinal center cells.
GILT/IFI30 Antibody

Western Blot-GILT/IFI30 Antibody-NBP1-83366-

Western Blot-GILT/IFI30 Antibody-NBP1-83366-Analysis in human cell line RPMI-8226.

Applications for GILT/IFI30 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25 - 2 ug/mL

Immunohistochemistry

1:2500 - 1:5000

Immunohistochemistry-Paraffin

1:2500 - 1:5000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Reviewed Applications

Read 1 review rated 5 using NBP1-83366 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2), 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GILT/IFI30

IFI30 is encoded by this gene is a lysosomal thiol reductase that at low pH can reduce protein disulfide bonds. The enzyme is expressed constitutively in antigen-presenting cells and induced by gamma-interferon in other cell types. This enzyme has an important role in MHC class II-restricted antigen processing. [provided by RefSeq]

Long Name

Gamma-Interferon-inducible Lysosomal Thiol Reductase

Alternate Names

IFI30, IP30, Legumaturain

Gene Symbol

IFI30

Additional GILT/IFI30 Products

Product Documents for GILT/IFI30 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GILT/IFI30 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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