GilZ Antibody (CFMKG15) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-00143
![Western Blot: GilZ Antibody (CFMKG15)BSA Free [NBP2-00143]](https://resources.bio-techne.com/images/products/GilZ-Antibody-CFMKG15-Western-Blot-NBP2-00143-img0001.jpg "Western Blot: GilZ Antibody (CFMKG15)BSA Free [NBP2-00143]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2a Kappa Clone # CFMKG15
Format
BSA Free
Concentration
0.5 mg/ml
Product Specifications
Immunogen
The immunogen for this antibody is GILZ.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2a Kappa
Scientific Data Images for GilZ Antibody (CFMKG15) - BSA Free
Western Blot: GilZ Antibody (CFMKG15)BSA Free [NBP2-00143]
Western Blot: GilZ Antibody (CFMKG15) [NBP2-00143] - Left: Mouse thymocytes cultured without (lane 1) or with (lane 2) dexamethasone (10e-7 M) for 3 hr. The membrane was probed with 10 ug/mL of Anti-Human/Mouse GILZ Purified, followed by Anti-Rat IgG HRP. Right: Normal human peripheral blood cells cultured without (lane 1) or with (lane 2) dexamethasone (10e-7 M) for 3 hours. The membrane was probed with 2 ug/mL of Anti-Human/Mouse GILZ Purified, followed by Anti-Rat IgG HRP.![Flow Cytometry: GilZ Antibody (CFMKG15) - BSA Free [NBP2-00143]](https://resources.bio-techne.com/images/products/GilZ-Antibody-CFMKG15-Flow-Cytometry-NBP2-00143-img0003.jpg "Flow Cytometry: GilZ Antibody (CFMKG15) - BSA Free [NBP2-00143]")
Flow Cytometry: GilZ Antibody (CFMKG15) - BSA Free [NBP2-00143]
Flow Cytometry: GilZ Antibody (CFMKG15) [NBP2-00143] - Analysis using the PE conjugate of NBP2-00143. C57BL/6 lymph node cells were cultured for 3 hr with (purple histogram) or without (blue histogram) dexamethasone, followed by intracellular staining with 0.25 ug of Rat IgG2a kappa Isotype Control PE (filled histogram) or 0.25 ug of Anti-Human/Mouse GILZ PE (filled histograms). CD3+ cells in the lymphocyte gate were used for analysis.Applications for GilZ Antibody (CFMKG15) - BSA Free
Application
Recommended Usage
Western Blot
1:100-1:2000
Application Notes
This CFMKG15 antibody has been tested by western blot analysis of dexamethasone-treated mouse thymocytes. CFMKG15 recognizes endogenous mouse GILZ with an approximate molecular weight of 14 kDa; a non-specific band of approximately 95 kDa is also observed. CFMKG15 also recognizes endogenous human GILZ in dexamethasone-treated peripheral blood mononuclear cells, with an approximate molecular weight of 14 kDa; non-specific bands may also appear at approximately 33 kDa and 95 kDa. This can be used at less than or equal to 2 ug/mL. This CFMKG15 antibody has also been reported for use in immunocytochemistry. (Fluorochrome conjugated CFMKG15 is recommended for use in intracellular flow cytometry) It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS (pH 7.2)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: GilZ
Alternate Names
Delta sleep-inducing peptide immunoreactor, DSIP-immunoreactive leucine zipper protein, DSIP-immunoreactive peptide, GILZDKFZp313A1123, Glucocorticoid-induced leucine zipper protein, Protein DIP, TSC22 domain family protein 3, TSC22 domain family, member 3, TSC-22 related protein, TSC-22-like protein, TSC-22-related protein, TSC-22Rimmunoreactor
Gene Symbol
TSC22D3
Additional GilZ Products
Product Documents for GilZ Antibody (CFMKG15) - BSA Free
Product Specific Notices for GilZ Antibody (CFMKG15) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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