GIPR Antibody (Gipg013) - Chimeric - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-81068

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine

Applications

Block/Neutralize, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Lambda Clone # Gipg013

Format

Azide and BSA Free

Concentration

1 mg/ml

View all GIPR Antibodies »

Product Summary for GIPR Antibody (Gipg013) - Chimeric - Azide and BSA Free

Immunogen

Human GIPR.

Specificity

The original has a Ki value of 7 nM and a Kd value of 6.8 nM for human GIPR.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Lambda

Scientific Data Images for GIPR Antibody (Gipg013) - Chimeric - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: GIPR Antibody (Gipg013) - Chimeric - Azide and BSA Free [NBP2-81068]

Immunocytochemistry/ Immunofluorescence: GIPR Antibody (Gipg013) - Chimeric - Azide and BSA Free [NBP2-81068]

Immunocytochemistry/Immunofluorescence: GIPR Antibody (Gipg013) - Chimeric [NBP2-81068] - Immunofluoresence staining of fixed MDA-MB-231 cells with GIPR Antibody (Gipg013) [NBP2-81068]. Immunofluorescence analysis of paraformaldehyde fixed MDA-MB-231 cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of Gipg013 [NBP2-81068] at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing cytoplasmic staining with additional puncta. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81068, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.

Applications for GIPR Antibody (Gipg013) - Chimeric - Azide and BSA Free

Application
Recommended Usage

Block/Neutralize

Optimal dilutions of this antibody should be experimentally determined.

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This full-length, chimeric rabbit antibody was made using the variable domain sequences of the original Mouse scFv format, for improved compatibility with existing reagents, assays and techniques. This antibody binds to GIPr, a GPCR expressed on pancreatic beta-cells, where activation leads to the release of insulin. Endogeonous ligands for the receptor include oleylethanolamide and lysophosphatidylcholine. The antibody acts as a competitive antagonist. In rat islets, the antibody inhibits GIP-induced secretion of insulin by up to 81%.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: GIPR

GIPR, or Gastric Inhibitory Polypeptide Receptor, mediates GIP-induced secretion of insulin by pancreatic islet beta cells after a meal. GIP is a gastrointestinal peptide hormone of 42 aa that is released from duodenal endocrine K cells after absorption of glucose or fat. Stimulation of the GIPR on pancreatic cells activates adenylyl cyclase and mitogen-activated protein kinase, resulting in increased insulin secretion. Mice with a targeted mutation of GIPR have higher blood glucose levels with impaired initial insulin response after oral glucose load. Analysis of GIPR knockout mice suggest that GIPR defects may contribute to the pathogenesis of diabetes and obesity. Ectopic expression of functional GIPR and its coupling to steroidogenesis has been suggested to be the main cause of food-dependent Cushing's syndrome. Two isoforms of GIPR are produced by alternative splicing. GIPR expression has been reported in human bone, fetal adrenal, and pancreas. Little expression has been identified in normal adult adrenal, but overexpression of GIPR has been observed in the adrenal in food-dependent Cushing's syndrome. GIPR expression has been identified in rat brain, heart, pancreas, and small intestine. ESTs have been isolated from colon libraries.

Long Name

Gastric Inhibitory Polypeptide Receptor

Alternate Names

PGQTL2

Gene Symbol

GIPR

Additional GIPR Products

Product Documents for GIPR Antibody (Gipg013) - Chimeric - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for GIPR Antibody (Gipg013) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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