GIT2 Antibody (3B5-B9)

Novus Biologicals, part of Bio-Techne | Catalog # H00009815-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3B5-B9

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all GIT2 Antibodies »

Product Specifications

Immunogen

GIT2 (AAH01379, 1 a.a. ~ 471 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSKRLRSSEVCADCSGPDPSWASVNRGTFLCDECCSVHRSLGRHISQVRHLKHTPWPPTLLQMVETLYNNGANSIWEHSLLDPASIMSGRRKANPQDKVHPNKAEFIRAKYQMLAFVHRLPCRDDDSVTAKDLSKQLHSSVRTGNLETCLRLLSLGAQANFFHPEKGNTPLHVASKAGQILQAELLAVYGADPGTQDSSGKTPVDYARQGGHHELAERLVEIQYELTDRLAFYLCGRKPDHKNGQHFIIPQMADSSLDLSELAKAAKKKLQSLSNHLFEELAMDMYDEVDRRETDAVWLATQNHSALVTETTVVPFLPVNPEYSSTRNQGRQKLARFNAHEFATLVIDILSDAKRRQQGSSLSGSKDNVELILKTINNQHSVESQDNDQPDYDSVASDEDTDLETTASKTNRQKSLDSDLSDGPVTVQEFMEVKNALVASEAKIQQLMKVNNNLSDELRIMQKKLLGKDAN

Reactivity Notes

Human. Other species not tested.

Specificity

GIT2 - G protein-coupled receptor kinase interactor 2

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for GIT2 Antibody (3B5-B9)

Western Blot: GIT2 Antibody (3B5-B9) [H00009815-M01]

Western Blot: GIT2 Antibody (3B5-B9) [H00009815-M01]

Western Blot: GIT2 Antibody (3B5-B9) [H00009815-M01] - GIT2 monoclonal antibody (M01), clone 3B5-B9 Analysis of GIT2 expression in Jurkat.
Immunocytochemistry/ Immunofluorescence: GIT2 Antibody (3B5-B9) [H00009815-M01]

Immunocytochemistry/ Immunofluorescence: GIT2 Antibody (3B5-B9) [H00009815-M01]

Immunocytochemistry/Immunofluorescence: GIT2 Antibody (3B5-B9) [H00009815-M01] - Analysis of monoclonal antibody to GIT2 on HeLa cell. Antibody concentration 20 ug/ml.
Immunohistochemistry-Paraffin: GIT2 Antibody (3B5-B9) [H00009815-M01]

Immunohistochemistry-Paraffin: GIT2 Antibody (3B5-B9) [H00009815-M01]

Immunohistochemistry-Paraffin: GIT2 Antibody (3B5-B9) [H00009815-M01] - Analysis of monoclonal antibody to GIT2 on formalin-fixed paraffin-embedded human lung. Antibody concentration 3 ug/ml.
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Applications for GIT2 Antibody (3B5-B9)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: GIT2

This gene encodes a member of the GIT protein family. GIT proteins interact with G protein-coupled receptor kinases and possess ADP-ribosylation factor (ARF) GTPase-activating protein (GAP) activity. This gene undergoes extensive alternative splicing; although ten transcript variants have been described, the full length sequence has been determined for only four variants. The various isoforms have functional differences, with respect to ARF GAP activity and to G protein-coupled receptor kinase 2 binding.

Alternate Names

ARF GAP GIT2, ARF GTPase-activating protein GIT2, CAT2, CAT-2, cool-associated, tyrosine phosphorylated protein 2, Cool-interacting tyrosine-phosphorylated protein 2, DKFZp686G01261, G protein-coupled receptor kinase interacting ArfGAP 2, G protein-coupled receptor kinase interactor 2, G protein-coupled receptor kinase-interactor 2, KIAA0148GRK-interacting protein 2, MGC760

Entrez Gene IDs

9815 (Human)

Gene Symbol

GIT2

OMIM

608564 (Human)

UniProt

AAH01379 (Human)

Additional GIT2 Products

Product Documents for GIT2 Antibody (3B5-B9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GIT2 Antibody (3B5-B9)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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