GITR/TNFRSF18 Antibody (YGITR765) - Chimeric - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-81090

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Mouse

Applications

Block/Neutralize, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # YGITR765

Format

Azide and BSA Free

Concentration

1 mg/ml

View all GITR/TNFRSF18 Antibodies »

Product Specifications

Immunogen

nB2/6TG cells transfected with mouse GITR/TNFRSF18 injected into DA rats.

Specificity

This antibody binds murine GITR/TNFRSF18.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for GITR/TNFRSF18 Antibody (YGITR765) - Chimeric - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: GITR/TNFRSF18 Antibody (YGITR765) - Chimeric - Azide and BSA Free [NBP2-81090]

Immunocytochemistry/ Immunofluorescence: GITR/TNFRSF18 Antibody (YGITR765) - Chimeric - Azide and BSA Free [NBP2-81090]

Immunocytochemistry/Immunofluorescence: GITR/TNFRSF18 Antibody (YGITR765) [NBP2-81090] - Immunofluorescence staining of fixed mouse splenocytes with GITR/TNFRSF18 Antibody (YGITR765) [NBP2-81090]. Immunofluorescence analysis of paraformaldehyde fixed mouse (Mus musculus) splenocytes on Shi-fix coverslips, permeabilized with 0.15% Triton stained with the chimeric rabbit version of YGITR765 [NBP2-81090] at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81090, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.
Flow Cytometry: GITR/TNFRSF18 Antibody (YGITR765) - Chimeric - Azide and BSA Free [NBP2-81090]

Flow Cytometry: GITR/TNFRSF18 Antibody (YGITR765) - Chimeric - Azide and BSA Free [NBP2-81090]

Flow Cytometry: GITR/TNFRSF18 Antibody (YGITR765) [NBP2-81090] - Flow-cytometry using [NBP2-81090]. Mouse activated T cells were stained with an isotype control (NBP2-52638, Orange) or the rabbit-chimeric version of YGITR765 [NBP2-81090] at a concentration of 1 ug/ml for 30 mins at RT without (Blue) and with (Grey) stimulation of the lymphocytes with immobilized anti-CD3 and anti-CD28 antibodies. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.

Applications for GITR/TNFRSF18 Antibody (YGITR765) - Chimeric - Azide and BSA Free

Application
Recommended Usage

Block/Neutralize

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2b format, for improved compatibility with existing reagents, assays and techniques. A chimeric version of this antibody featuring a human IgG1 Fc-portion engineered to lack the N-linked glycosylation site at amino acid 297 was shown to enhance humoral immune responses by blocking GITR signallin (Ponte 2010).

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: GITR/TNFRSF18

GITR is encoded by this gene is a member of the TNF-receptor superfamily. This receptor has been shown to have increased expression upon T-cell activation, and it is thought to play a key role in dominant immunological self-tolerance maintained by CD25(+)CD4(+) regulatory T cells. Knockout studies in mice also suggest the role of this receptor is in the regulation of CD3-driven T-cell activation and programmed cell death. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq]

Long Name

Glucocorticoid Induced TNF Receptor Family Related Gene

Alternate Names

AITR, CD357, TNFRSF18

Gene Symbol

TNFRSF18

Additional GITR/TNFRSF18 Products

Product Documents for GITR/TNFRSF18 Antibody (YGITR765) - Chimeric - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GITR/TNFRSF18 Antibody (YGITR765) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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