GL7 (T and B Cell Activation Marker) Antibody (GL-7)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-00362
![Flow Cytometry: GL7 (T and B Cell Activation Marker) Antibody (GL-7) [NBP2-00362]](https://resources.bio-techne.com/images/products/GL7-T-and-B-Cell-Activation-Marker-Antibody-GL-7-Flow-Cytometry-NBP2-00362-img0003.jpg "Flow Cytometry: GL7 (T and B Cell Activation Marker) Antibody (GL-7) [NBP2-00362]")
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Applications
Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Rat IgM Clone # GL-7
Concentration
0.5 mg/ml
Product Summary for GL7 (T and B Cell Activation Marker) Antibody (GL-7)
Immunogen
The immunogen for this antibody is T- and B-cell activation antigen (GL7, Ly-77).
Clonality
Monoclonal
Host
Rat
Isotype
IgM
Scientific Data Images for GL7 (T and B Cell Activation Marker) Antibody (GL-7)
Flow Cytometry: GL7 (T and B Cell Activation Marker) Antibody (GL-7) [NBP2-00362]
Flow Cytometry: GL7 (T and B Cell Activation Marker) Antibody (GL-7) [NBP2-00362] - Analysis using the Biotin conjugate of NBP2-00362. Staining of 3-day unstimulated (left) and 3-day ConA activated (right) BALB/c splenocytes with 0.5 ug of Rat IgM Isotype Control Biotin (open histogram) or 0.25ug of Anti-Human/Mouse GL7 (T and B Cell Activation Marker) Biotin (filled histogram) followed by Streptavidin PE. Total viable cells were used for analysis.![Flow Cytometry: GL7 (T and B Cell Activation Marker) Antibody (GL-7) [NBP2-00362]](https://resources.bio-techne.com/images/products/GL7-T-and-B-Cell-Activation-Marker-Antibody-GL-7-Flow-Cytometry-NBP2-00362-img0001.jpg "Flow Cytometry: GL7 (T and B Cell Activation Marker) Antibody (GL-7) [NBP2-00362]")
Flow Cytometry: GL7 (T and B Cell Activation Marker) Antibody (GL-7) [NBP2-00362]
Flow Cytometry: GL7 (T and B Cell Activation Marker) Antibody (GL-7) [NBP2-00362] - Staining of 3-day unstimulated (left) and 3-day ConA-stimulated (right) BALB/c splenocytes with 0.25 ug of Rat IgM kappa Isotype Control Purified (open histogram) or 0.25 ug of Anti-Human/Mouse GL7 (T and B Cell Activation Marker) Purified (filled histogram) followed by Anti-Rat IgM PE.Applications for GL7 (T and B Cell Activation Marker) Antibody (GL-7)
Application
Recommended Usage
Flow Cytometry
< = 0.5 ug/10^6 cells in 100 ul
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:10-1:500
Immunoprecipitation
1:10-1:500
Application Notes
The GL-7 (GL7) antibody has been tested by flow cytometric analysis of ConA-activated mouse splenocytes. This can be used at less than or equal to 0.5 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. The GL-7 antibody has also been reported for use in immunoprecipitation and immunohistochemical staining of frozen tissue sections. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2)
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: GL7 (T and B Cell Activation Marker)
Additional GL7 (T and B Cell Activation Marker) Products
Product Documents for GL7 (T and B Cell Activation Marker) Antibody (GL-7)
Product Specific Notices for GL7 (T and B Cell Activation Marker) Antibody (GL-7)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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