GLIS3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33787

Novus Biologicals, part of Bio-Techne
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NBP2-33787
NBP2-33787-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all GLIS3 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: SPGHNVQGSPHNPSSQLPPLTAVDAGAERFAPSAPSPHHISPRRVPAPSSILQRTQPPYTQQPSGSHLKSYQPETNSSFQPNGI

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (83%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GLIS3 Antibody

Immunocytochemistry/ Immunofluorescence: GLIS3 Antibody [NBP2-33787]

Immunocytochemistry/ Immunofluorescence: GLIS3 Antibody [NBP2-33787]

Immunocytochemistry/Immunofluorescence: GLIS3 Antibody [NBP2-33787] - Staining of human cell line A549 shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: GLIS3 Antibody [NBP2-33787]

Immunohistochemistry-Paraffin: GLIS3 Antibody [NBP2-33787]

Immunohistochemistry-Paraffin: GLIS3 Antibody [NBP2-33787] - Staining of human thyroid gland shows moderate nuclear positivity in glandular cells.

Applications for GLIS3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GLIS3

The GLIS3 gene is a member of the GLI-similar zinc finger protein family and encodes a nuclear protein with five C2H2-typezinc finger domains. This protein functions as both a repressor and activator of transcription and is specificallyinvolved in the developm

Alternate Names

FLJ38999, FLJ90578, GLIS family zinc finger 3, GLI-similar 3, OK/KNS-cl.4, zinc finger protein 515, zinc finger protein GLIS3

Gene Symbol

GLIS3

UniProt

Q8NEA6 (Human)

Additional GLIS3 Products

Product Documents for GLIS3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for GLIS3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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