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GLP/EHMT1 Antibody (3G1)

Novus Biologicals, part of Bio-Techne | Catalog # H00079813-M05

Novus Biologicals, part of Bio-Techne
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H00079813-M05

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3G1

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

EHMT1 (NP_079033, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAADEGSAEKQAGEAHMAADGETNGSCENSDASSHANAAKHTQDSARVNPQDGTNTLTRIAENGVSERDSEAAKQNHVTADDFVQTSVIGSNGYILNKPA

Specificity

EHMT1 - euchromatic histone-lysine N-methyltransferase 1 (3G1)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for GLP/EHMT1 Antibody (3G1)

Western Blot: GLP/EHMT1 Antibody (3G1) [H00079813-M05]

Western Blot: GLP/EHMT1 Antibody (3G1) [H00079813-M05]

Western Blot: GLP/EHMT1 Antibody (3G1) [H00079813-M05] - Analysis of EHMT1 expression in Hela S3 NE (Cat # L013V3).
Immunocytochemistry/ Immunofluorescence: GLP/EHMT1 Antibody (3G1) [H00079813-M05]

Immunocytochemistry/ Immunofluorescence: GLP/EHMT1 Antibody (3G1) [H00079813-M05]

Immunocytochemistry/Immunofluorescence: GLP/EHMT1 Antibody (3G1) [H00079813-M05] - Analysis of monoclonal antibody to EHMT1 on HeLa cell. Antibody concentration 10 ug/ml

Applications for GLP/EHMT1 Antibody (3G1)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: GLP/EHMT1

Euchromatic histone-lysine N-methyltransferase 1 (EHMT1) is a histone methyltransferase that catalyzes the methylation of lysine-9 of histone H3 (H3-K9). H3-K9 histone methylation is restricted to euchromatin and functions to epigenetically silence gene transcription. Loss of function mutations in EHMT1 cause the 9q34 subtelomeric deletion syndrome, a syndrome characterized by severe mental retardation, hypotonia, brachy(micro)cephaly, heart defects, and distinct facial anomalies. Alternate names for EHMT1 include histone H3-K9 methyltransferase 5, H3-K9-HMTase5, eu-HMTase, G9a-like protein 1, GLP1, lysine N-methyltransferase 1D, EUHMTASE1, KMT1D, and KIAA1876.

Long Name

G9a-like Protein 1/Euchromatic Histone-Lysine N-Methyltransferase 1

Alternate Names

DEL9q34, EHMT1, Eu-HMTase1, KMT1D, Lysine N-methyltransferase 1D

Entrez Gene IDs

79813 (Human)

Gene Symbol

EHMT1

Additional GLP/EHMT1 Products

Product Documents for GLP/EHMT1 Antibody (3G1)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GLP/EHMT1 Antibody (3G1)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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