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GLP/EHMT1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18679

Novus Biologicals, part of Bio-Techne
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NBP3-18679-100ul
NBP3-18679-25ul

Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Polyclonal antibody raised in rabbit against mouse GLP/EHMT1 (euchromatic histone-lysine N-methyltransferase 1), using three KLH-conjugated synthetic peptides containing a sequence from the central part of the protein (1).

Clonality

Polyclonal

Host

Rabbit

Scientific Data Images for GLP/EHMT1 Antibody

Western Blot: GLP/EHMT1 Antibody [NBP3-18679]

Western Blot: GLP/EHMT1 Antibody [NBP3-18679]

Western Blot: GLP/EHMT1 Antibody [NBP3-18679] - Figure 2. Western blot analysis using the Diagenode antibody directed against EHMT1Nuclear extracts of HeLa cells (40 ug) were analysed by Western blot using the antibody against EHMT1 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest (expected size: 120 kDa) is indicated on the right; the marker (in kDa) is shown on the left. The smaller fragment of approximately 92 kDa may represent a splicing variant.
ELISA: GLP/EHMT1 Antibody [NBP3-18679]

ELISA: GLP/EHMT1 Antibody [NBP3-18679]

ELISA: GLP/EHMT1 Antibody [NBP3-18679] - Figure 1. Determination of the titerTo determine the titer, an ELISA was performed using a serial dilution of the antibody directed against human EHMT1. The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,000.

Applications for GLP/EHMT1 Antibody

Application
Recommended Usage

ELISA

1:100-1:200

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Whole Antiserum

Formulation

Whole antiserum

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: GLP/EHMT1

Euchromatic histone-lysine N-methyltransferase 1 (EHMT1) is a histone methyltransferase that catalyzes the methylation of lysine-9 of histone H3 (H3-K9). H3-K9 histone methylation is restricted to euchromatin and functions to epigenetically silence gene transcription. Loss of function mutations in EHMT1 cause the 9q34 subtelomeric deletion syndrome, a syndrome characterized by severe mental retardation, hypotonia, brachy(micro)cephaly, heart defects, and distinct facial anomalies. Alternate names for EHMT1 include histone H3-K9 methyltransferase 5, H3-K9-HMTase5, eu-HMTase, G9a-like protein 1, GLP1, lysine N-methyltransferase 1D, EUHMTASE1, KMT1D, and KIAA1876.

Long Name

G9a-like Protein 1/Euchromatic Histone-Lysine N-Methyltransferase 1

Alternate Names

DEL9q34, EHMT1, Eu-HMTase1, KMT1D, Lysine N-methyltransferase 1D

Gene Symbol

EHMT1

UniProt

Additional GLP/EHMT1 Products

Product Documents for GLP/EHMT1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GLP/EHMT1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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