Glucokinase/GCK Antibody (4K9D5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16579

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16579-100ul
NBP3-16579-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4K9D5

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Glucokinase/GCK Antibodies »

Product Summary for Glucokinase/GCK Antibody (4K9D5)

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 271-421 of human Glucokinase/GCK (P35557). LEYDRLVDESSANPGQQLYEKLIGGKYMGELVRLVLLRLVDENLLFHGEASEQLRTRGAFETRFVSQVESDTGDRKQIYNILSTLGLRPSTTDCDIVRRACESVSTRAAHMCSAGLAGVINRMRESRSEDVMRITVGVDGSVYKLHPSFKE

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Glucokinase/GCK Antibody (4K9D5)

Western Blot: Glucokinase/GCK Antibody (4K9D5) [NBP3-16579]

Western Blot: Glucokinase/GCK Antibody (4K9D5) [NBP3-16579]

Western Blot: Glucokinase/GCK Antibody (4K9D5) [NBP3-16579] - Western blot analysis of extracts of various cell lines, using Glucokinase/GCK (GCK) Rabbit mAb (NBP3-16579) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Applications for Glucokinase/GCK Antibody (4K9D5)

Application
Recommended Usage

Immunoprecipitation

1:500 - 1:1000

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Glucokinase/GCK

Hexokinases phosphorylate hexose to form hexose 6-phosphate, the first step for hexose metabolism. There are four mammalian hexokinases (I, II, III and IV) and hexokinase IV is commonly known as glucokinase (GCK). Unlike hexokinase I, II and III, which have high affinity for glucose and are strongly inhibited by the product, glucose-6-phosphate, GCK has much lower affinity for glucose and is not inhibited by the product. Consequently, GCK has a Km for glucose of approximately 7 mM, which is 100 times higher than that of hexokinase I, II, and III. This unique enzymatic property of GCK allows it to respond to blood glucose levels and contribute to the maintenance of blood glucose levels within the normal physiological range of 4 mM to 6 mM. In the pancreatic islets, GCK serves as a glucose sensor to control insulin release in the beta cells, and to control glucagon release in the alpha cells. In hepatocytes, GCK responds to changes of ambient glucose levels by increasing or reducing glycogen synthesis. Mutations in GCK have been associated with non-insulin-dependent diabetes mellitus, maturity-onset diabetes of the young type 2, and hyperinsulinemia of infancy. The enzyme activity was measured using a phosphatase coupled kinase assay.

Long Name

Glucose Kinase

Alternate Names

FGQTL3, GCK, GLK, Hexokinase-4, Hexokinase-D, HHF3, HK4, HXKP, LGLK

Gene Symbol

GCK

Additional Glucokinase/GCK Products

Product Documents for Glucokinase/GCK Antibody (4K9D5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Glucokinase/GCK Antibody (4K9D5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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