Glucose 6 Phosphate Dehydrogenase Antibody

Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody [NB100-236] -

Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody [NB100-236] - Dysregulation of Antioxidant proteins in response to chronic endurance exercise in aged Nrf2−/− mice hearts. Antioxidant protein expression (GPX1, CAT, G6PD, & SOD1) were determined using immunoblotting with specific antibodies. The relative intensity signals were quantified using ImageJ software & normalized to GAPDH intensity & represented as histogram. Experiments were analyzed using one way ANOVA followed by Tukey multiple comparison tests & differences between the means were considered statistically significant if P < 0.05 (* vs. WT-SED, # vs. Nrf2−/−-SED, $ vs. WT CEE). Image collected & cropped by CiteAb from the following publication (http://journal.frontiersin.org/article/10.3389/fphys.2017.00268/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody [NB100-236] -

Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody [NB100-236] - Effect of prolonged moderate exercise on Nrf2/ARE-Antioxidants in the aging heart.Representative immunoblots of cytosolic extracts from the hearts of young & old mice under basal conditions & following 6-weeks of moderate exercise training (MET). Protein blots were probed with respective antibodies as indicated. Individual lanes represent separate animals (n = 4–6/group). A. Analysis of nuclear Nrf2 in young & old mice subjected to MET. In sedentary mice, Nrf2 protein levels were decreased significantly in old when compared to young (*p<0.05). Following MET, nuclear Nrf2 levels were significantly increased in old mice to levels equivalent to those of young mice (#, $p<0.05 in MET vs. respective basal). (B) Densitometry analysis of respective protein signals were performed using Image-J & expressed relative to mean values of the sedentary-young group. Under basal conditions, a significant decrease in the protein levels of GSR, G6PD, NQO1, catalase, GPX1 & GCS were observed in the heart tissues of old when compared to young mice. Following 6-weeks of moderate exercise, most of the antioxidants were significantly (*p<0.05) upregulated or stabilized in the aging heart. Image collected & cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0045697), licensed under a CC-BY license. Not internally tested by Novus Biologicals.