GluR1 [p Ser831] Antibody (JE44-96) - AMPA Subtype
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32767
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE44-96
Concentration
1 mg/ml
Product Summary for GluR1 [p Ser831] Antibody (JE44-96) - AMPA Subtype
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Ser831 of GluR1 (Uniprot: P42261)
Modification
p Ser 831
Localization
Cell membrane, Endoplasmic reticulum membrane, Cell junction, Cell projection.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
102 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for GluR1 [p Ser831] Antibody (JE44-96) - AMPA Subtype
Western Blot: GluR1 [p Ser831] Antibody (JE44-96) - AMPA Subtype [NBP3-32767]
Western Blot: GluR1 [p Ser831] Antibody (JE44-96) - AMPA Subtype [NBP3-32767] - Western blot analysis of GluR1 on different lysates with Rabbit anti-GluR1 antibody (NBP3-32767) at 1/1,000 dilution.Lane 1: Mouse brain tissue lysate
Lane 2: Human brain tissue lysate
Lysates/proteins at 30 ug/Lane.
Predicted band size: 102 kDa
Observed band size: 110 kDa
Exposure time: 1 minute;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32767) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Applications for GluR1 [p Ser831] Antibody (JE44-96) - AMPA Subtype
Application
Recommended Usage
Western Blot
1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: GluR1
Long Name
Glutamate Receptor 1
Alternate Names
GluA1, GLURA, GRIA1
Gene Symbol
GRIA1
Additional GluR1 Products
Product Documents for GluR1 [p Ser831] Antibody (JE44-96) - AMPA Subtype
Product Specific Notices for GluR1 [p Ser831] Antibody (JE44-96) - AMPA Subtype
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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