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GluR1 [p Ser831] Antibody (JE44-96) - AMPA Subtype

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32767

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-32767

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JE44-96

Concentration

1 mg/ml

Product Summary for GluR1 [p Ser831] Antibody (JE44-96) - AMPA Subtype

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Ser831 of GluR1 (Uniprot: P42261)

Modification

p Ser 831

Localization

Cell membrane, Endoplasmic reticulum membrane, Cell junction, Cell projection.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

102 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GluR1 [p Ser831] Antibody (JE44-96) - AMPA Subtype

GluR1 [p Ser831] Antibody (JE44-96) - AMPA Subtype

Western Blot: GluR1 [p Ser831] Antibody (JE44-96) - AMPA Subtype [NBP3-32767]

Western Blot: GluR1 [p Ser831] Antibody (JE44-96) - AMPA Subtype [NBP3-32767] - Western blot analysis of GluR1 on different lysates with Rabbit anti-GluR1 antibody (NBP3-32767) at 1/1,000 dilution.

Lane 1: Mouse brain tissue lysate
Lane 2: Human brain tissue lysate

Lysates/proteins at 30 ug/Lane.

Predicted band size: 102 kDa
Observed band size: 110 kDa

Exposure time: 1 minute;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32767) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Applications for GluR1 [p Ser831] Antibody (JE44-96) - AMPA Subtype

Application
Recommended Usage

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

1*TBS (pH7.4), 0.05% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GluR1

Glutamate Receptor 1 belongs to a family of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptors. Alternatively spliced transcript variants encoding different isoforms have been found for Glutamate Receptor 1. Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The classification of glutamate receptors is based on their activation by different pharmacologic agonists.

Long Name

Glutamate Receptor 1

Alternate Names

GluA1, GLURA, GRIA1

Gene Symbol

GRIA1

Additional GluR1 Products

Product Documents for GluR1 [p Ser831] Antibody (JE44-96) - AMPA Subtype

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GluR1 [p Ser831] Antibody (JE44-96) - AMPA Subtype

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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