GluR2/3 Antibody (JE33-28)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32478
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE33-28
Concentration
1 mg/ml
Product Summary for GluR2/3 Antibody (JE33-28)
Immunogen
Synthetic peptide within Human GRIA2 aa 834-883 / 883. (Uniprot: P42262)
Localization
Cell membrane, Endoplasmic reticulum membrane, Postsynaptic cell membrane, Postsynaptic density membrane.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
99 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for GluR2/3 Antibody (JE33-28)
Western Blot: GluR2/3 Antibody (JE33-28) [NBP3-32478]
Western Blot: GluR2/3 Antibody (JE33-28) [NBP3-32478] - Western blot analysis of GluR2/3 on different lysates with Rabbit anti-GluR2/3 antibody (NBP3-32478) at 1/1,000 dilution.Lane 1: Mouse brain tissue lysate
Lane 2: Rat brain tissue lysate
Lysates/proteins at 20 ug/Lane.
Predicted band size: 99 kDa
Observed band size: 99 kDa
Exposure time: 1 minute;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32478) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.
Applications for GluR2/3 Antibody (JE33-28)
Application
Recommended Usage
Western Blot
1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: GluR2/3
Long Name
Glutamate Receptor 2/3
Alternate Names
AMPA 2, glutamate receptor, ionotropic, AMPA 2
Gene Symbol
GRIA2
Additional GluR2/3 Products
Product Documents for GluR2/3 Antibody (JE33-28)
Product Specific Notices for GluR2/3 Antibody (JE33-28)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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