NBP1-49533 and NBP1-49533R are listed as being raised against a synthetic peptide made to an internal portion of the human GLUT4 protein (between residues 480-509 or 480-530 for NBP1-49533R), but since the protein is only 509aa in length, I am curious to know if the description as internal means that the actual sequence does not include the very c-terminal part, or if the listed sequence range is incorrect and the actual sequence in more internal?

We highly appreciate your feedback on the immunogen info on the datasheet of our Glucose Transporter GLUT4 antibody #NBP1-49533, and yes, the immunogen sequence is from human Glucose Transporter GLUT4 protein between residues 480-509. Similarly, the sequence for Glucose Transporter GLUT4 Antibody [DyLight 550] (NBP1-49533R) would also be the same (between residues 480-509). It is certainly C-terminal region and we apologize for the mistake committed on our end about writing it as internal region.

Do you happen to sell peptide for this antibody?

We do have a peptide for this particular product. The catalog number is NBP1-49533PEP.

Would you please help confirm if NBP1-49533 has been tested for IHC with frozen sections?

NBP1-49533 has been validated in WB, ICC/IF and IHC-P, but has not been validated in IHC-Fr yet. However, because this antibody worked very well in ICC/IF, I strongly believe that it will work in IHC-Fr also. Please see the protocol for IHC-Fr which is utilized in our lab.

NBP1-49533 and NBP1-49533R are listed as being raised against a synthetic peptide made to an internal portion of the human GLUT4 protein (between residues 480-509 or 480-530 for NBP1-49533R), but since the protein is only 509aa in length, I am curious to know if the description as "internal" means that the actual sequence does not include the very c-terminal part, or if the listed sequence range is incorrect and the actual sequence in more internal?

We highly appreciate your feedback on the immunogen info on the datasheet of our Glucose Transporter GLUT4 antibody #NBP1-49533, and yes, the immunogen sequence is from human Glucose Transporter GLUT4 protein between residues 480-509. Similarly, the sequence for Glucose Transporter GLUT4 Antibody [DyLight 550] (NBP1-49533R) would also be the same (between residues 480-509). It is certainly C-terminal region and we apologize for the mistake committed on our end about writing it as "internal" region.

Regarding NBP1-49533AF647, Glut4-Alexa Fluor 647, the data sheet declares that the immunogen is aa480-509. Uniport says it’s a cytoplasmatic region of the protein. Is this antibody suitable for flow? Has it been validated for this application? Can you provide the suggested protocol, specifically the fixation and permeabilzation part? Any available references for this antibody and application?

This antibody was validated in flow cytometry. We used our intracellular flow protocol found here. The results from this testing can be seen on the unconjugated part datasheet here

Regarding the use of NBP1-49533, is this anti Glut4 Antibody validated for extracellular domain of Glut4 in for flow cytometry? The data sheet shows images if intracellular and additional images of flow images that are not specified.

NBP1-49533 is not specific for the extracellular domain of Glut4 becuase the immunogen of the antibody pertains to the cytoplasmic C-terminus of Glut4: Immunogen: A synthetic peptide made to a C-terminal portion of the human Glucose Transporter GLUT4 protein (between residues 480-509) [UniProt P14672]. Amino acids 480-509 correspond to the cytoplasmic tail of this protein, accordingly, NBP1-49533 has only been used for labeling the intracellular staining in FLOW cytometry. The only other option we have is MAB86541, which is made by our sister company R&D systems. They used their protocol for "Staining Membrane-associated Proteins" to product the Flow results, so if you have any other questions abotu this product I would contact them for the most accurate and proper information: https://www.rndsystems.com/resources/protocols/flow-cytometry-protocol-staining-membrane-associated-proteins-suspended-cells

Glut4 Antibody - BSA Free

Name: Glut4 Antibody - BSA Free
Brand: Novus Biologicals
SKU: NBP1-49533
Rating: 4 (4 reviews)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-49533

(4)

Citations (42)

Product Datasheet / COA / SDS / Protocols & Manuals

View Available Conjugates

Conjugate

Catalog #

Alexa Fluor 350

NBP1-49533AF350

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Alexa Fluor 405

NBP1-49533AF405

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Alexa Fluor 488

NBP1-49533AF488

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Alexa Fluor 532

NBP1-49533AF532

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Alexa Fluor 594

NBP1-49533AF594

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Alexa Fluor 647

NBP1-49533AF647

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Alexa Fluor 700

NBP1-49533AF700

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Alexa Fluor 750

NBP1-49533AF750

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Allophycocyanin

NBP1-49533APC

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Allophycocyanin/Cy7

NBP1-49533APCCY7

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Biotin

NBP1-49533B

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CoraFluor 1

NBP1-49533CL1

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DyLight 350

NBP1-49533UV

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DyLight 405

NBP1-49533V

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DyLight 488

NBP1-49533G

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DyLight 550

NBP1-49533R

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DyLight 594

NBP1-49533DL594

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DyLight 650

NBP1-49533C

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DyLight 680

NBP1-49533FR

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DyLight 755

NBP1-49533IR

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FITC

NBP1-49533F

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HRP

NBP1-49533H

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Janelia Fluor 525

NBP1-49533JF525

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Janelia Fluor 549

NBP1-49533JF549

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Janelia Fluor 585

NBP1-49533JF585

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Janelia Fluor 635

NBP1-49533JF635

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Janelia Fluor 646

NBP1-49533JF646

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Janelia Fluor 669

NBP1-49533JF669

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mFluor Violet 450 SE

NBP1-49533MFV450

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mFluor Violet 500 SE

NBP1-49533MFV500

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mFluor Violet 610 SE

NBP1-49533MFV610

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NBP1-49533PE

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PE/Atto594

NBP1-49533PEATT594

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PE/Cy5.5

NBP1-49533PECY55

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PE/Cy7

NBP1-49533PECY7

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PerCP

NBP1-49533PCP

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Plant

Cited:

Human, Mouse, Rat, Fish, Plant

Applications

Validated:

Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Cited:

Flow Cytometry, IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Knockdown Validated, SDS-Page, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all Glut4 Antibodies »

Product Specifications

Immunogen

A synthetic peptide from the human Glucose Transporter GLUT4 protein [UniProt P14672]

Reactivity Notes

Plant reactivity reported in scientific literature (PMID: 31555030).

Localization

Endomembrane system; Multi-pass membrane protein. Cytoplasm - perinuclear region. Note: Localizes primarily to the perinuclear region, undergoing continued recycling to the plasma membrane where it is rapidly reinternalized. The dileucine internalization

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Glut4 Antibody - BSA Free

Western Blot: Glut4 AntibodyBSA Free [NBP1-49533]

Western Blot: Glut4 Antibody [NBP1-49533] - Glycolysis-related genes and proteins were upregulated in differentiated podocytes. Representative blot images of glycolysis-related proteins (n =3). d All proteins were normalized to tubulin and compared to UDPs. *P < 0.05, **P < 0.01, determined by t test. Data are shown as the means +/- SD. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41419-020-2481-5), licensed under a CC-BY license.

Immunocytochemistry/ Immunofluorescence: Glut4 Antibody - BSA Free [NBP1-49533]

Immunocytochemistry/Immunofluorescence: Glut4 Antibody [NBP1-49533] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-GLUT4 [NBP1-49533] at a 1:200 dilution overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunohistochemistry: Glut4 Antibody - BSA Free [NBP1-49533]

Immunohistochemistry: Glut4 Antibody [NBP1-49533] - Analysis of GLUT4 in mouse kidney

Flow Cytometry: Glut4 Antibody - BSA Free [NBP1-49533]

Flow Cytometry: Glut4 Antibody - BSA Free [NBP1-49533] - Analysis using the Alexa Fluor (R) 647 conjugate of NBP1-49533 (NBP1-49533AF647) on mouse Thymic Epithelial cells. Glut4 antibody in comparison to negative and IgG Control. Primary antibody dilution: 1:500. Image from verified customer review.

Western Blot: Glut4 AntibodyBSA Free [NBP1-49533]

Western Blot: Glut4 Antibody [NBP1-49533] - Analysis of GLUT4 in A) MCF7 whole cell lysate and B) 3T3L1 whole cell lysate.

Western Blot: Glut4 AntibodyBSA Free [NBP1-49533]

Western Blot: Glut4 Antibody [NBP1-49533] - Total protein from Human HeLa and A431, Mouse 3T3 and Rat PC12 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Glut4 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.

Western Blot: Glut4 AntibodyBSA Free [NBP1-49533]

Western Blot: Glut4 Antibody [NBP1-49533] - Total protein from 3T3-L1 mouse embryonic fibroblast adipose-like cell line, separated by 4-12% SDS-PAGE, transferred to nitrocellulose membrane and blocked in 5% non-fat milk for 1h at room temperature. The membrane was probed with anti-Glut4 0.5 ug/ml in non-fat milk. Undiffer: undifferentiated; Differ: Differentiated. Image from verified customer review.

Flow Cytometry: Glut4 Antibody - BSA Free [NBP1-49533]

Flow Cytometry: Glut4 Antibody [NBP1-49533] - GLUT4 Biotin/APC vs. [X] PE. Image from verified customer review.

Flow Cytometry: Glut4 Antibody - BSA Free [NBP1-49533]

Flow Cytometry: Glut4 Antibody [NBP1-49533] - Analysis using the Alexa Fluor (R) 647 conjugate of NBP1-49533. Staining of Glut 4 expression on Murine CD4+ T cells stimulated with anti-CD3/CD28 beads and insulin (1ug/mL) for 5 days in culture media with additional glucose provided. This Alexa Fluor (R) 647 conjugated Glut 4 antibody (orange) positively stained mouse CD4+ T cells compared to Isotype Control (Rb IgG AF647, Novus NBP2-36463AF647, blue) and fluorescence minus one/FMO control (red). Image from verified customer review.

Flow (Intracellular): Glut4 Antibody - BSA Free [NBP1-49533]

Flow (Intracellular): Glut4 Antibody [NBP1-49533] - An intracellular stain was performed on HepG2 with NBP1-49533 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody.

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Applications for Glut4 Antibody - BSA Free

Application

Recommended Usage

Flow Cytometry

reported by customer review

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:200

Immunohistochemistry-Paraffin

reported in scientific literature (PMID 24339864)

Knockdown Validated

reported in scientific literature (PMID 31555030)

Western Blot

0.5ug/ml

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 4 reviews rated 4 using NBP1-49533 in the following applications:

Western Blot (4 Reviews)

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Glut4

Glucose Transporter GLUT4 (SLC2A4 or GLUT4) is an insulin-sensitive glucose transporter which facilitate insulin-stimulated glucose uptake in adipose tissue, skeletal muscle and cardiac tissues (that specifically express GLUT4). GLUT4 is a 12-transmembrane domain/ multi-pass membrane protein localized in endomembrane system as well as cytoplasm near perinuclear region, and facilitates glucose transport in the direction of glucose gradient. This transporter localizes to intracellular organelles (endosomes) in unstimulated cells and translocates to the cell surface following insulin stimulation via TBC1D4 phosphorylation. Transgenic mice lacking or overexpressing GLUT4 respectively decrease or increase whole-body insulin sensitivity, suggesting its role in maintenance of glucose homeostasis, and accordingly, under obesity conditions, reduction of GLUT4 gene expression is directly related to insulin resistance development. Inflammatory cytokines produced by the adipose tissue including TNF-alpha, IL-6 etc have been related to reduce GLUT4 expression and defective GLUT4 is an underlying cause of NIDDM.

Alternate Names

SLC2A4

Entrez Gene IDs

6517 (Human); 20528 (Mouse)

Gene Symbol

SLC2A4

UniProt

P14672, P14142 (Mouse)

Additional Glut4 Products

Product Documents for Glut4 Antibody - BSA Free

Product Datasheets

Download Product Datasheets

Protocols & Manuals

Download ICC/IF Protocol

Download IHC-P Protocol

Download Western Blot Protocol

COA

SDS

Download SDS

Product Specific Notices for Glut4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

Protocols
FAQs

Glut4 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-49533

Key Product Details

Validated by

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

Format

Concentration

View all Glut4 Antibodies »

Product Specifications

Immunogen

Reactivity Notes

Localization

Clonality

Host

Isotype

Scientific Data Images for Glut4 Antibody - BSA Free

Western Blot: Glut4 AntibodyBSA Free [NBP1-49533]

Immunocytochemistry/ Immunofluorescence: Glut4 Antibody - BSA Free [NBP1-49533]

Immunohistochemistry: Glut4 Antibody - BSA Free [NBP1-49533]

Flow Cytometry: Glut4 Antibody - BSA Free [NBP1-49533]

Western Blot: Glut4 AntibodyBSA Free [NBP1-49533]

Western Blot: Glut4 AntibodyBSA Free [NBP1-49533]

Western Blot: Glut4 AntibodyBSA Free [NBP1-49533]

Flow Cytometry: Glut4 Antibody - BSA Free [NBP1-49533]

Flow Cytometry: Glut4 Antibody - BSA Free [NBP1-49533]

Flow (Intracellular): Glut4 Antibody - BSA Free [NBP1-49533]

Applications for Glut4 Antibody - BSA Free

Flow Cytometry

Immunocytochemistry/ Immunofluorescence

Immunohistochemistry

Immunohistochemistry-Paraffin

Knockdown Validated

Western Blot

Reviewed Applications

Formulation, Preparation, and Storage

Purification

Formulation

Format

Preservative

Concentration

Shipping

Stability & Storage

Background: Glut4

Alternate Names

Entrez Gene IDs

Gene Symbol

UniProt

Additional Glut4 Products

Product Documents for Glut4 Antibody - BSA Free

Product Datasheets

Protocols & Manuals

COA

Certificate of Analysis

SDS

Product Specific Notices for Glut4 Antibody - BSA Free