Glut4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-49840

Novus Biologicals, part of Bio-Techne
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NBP1-49840
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Glut4 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-TELEYLGPDEND corresponding to C-Terminus according to NP_001033.1.

Reactivity Notes

Expected from sequence similarity: Bovine, Porcine

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Glut4 Antibody

Western Blot: Glut4 Antibody [NBP1-49840]

Western Blot: Glut4 Antibody [NBP1-49840]

Western Blot: Glut4 Antibody [NBP1-49840] - Glucose Transporter GLUT4 expression in bovine skeletal muscle whole lysate. Image from verified customer review.
Immunohistochemistry-Paraffin: Glut4 Antibody [NBP1-49840]

Immunohistochemistry-Paraffin: Glut4 Antibody [NBP1-49840]

Immunohistochemistry-Paraffin: Glut4 Antibody [NBP1-49840] - 3.75ug/ml staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: Glut4 Antibody [NBP1-49840]

Western Blot: Glut4 Antibody [NBP1-49840]

Western Blot: Glut4 Antibody [NBP1-49840] - Glucose Transporter GLUT4 in Mouse Heart lysate (35ug protein in RIPA buffer) using NBP1-49840 at 1ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for Glut4 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

3.75 ug/ml

Peptide ELISA

Detection limit 1:16000

Western Blot

0.3-1 ug/ml
Application Notes
Western blot: Approx 45kDa band observed in Mouse and Rat Heart lysates (calculated MW of 54.8kDa according to Human NP_001033.1 and Mouse NP_033230.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Fischer et al, J Biol Chem. 1997 Mar 14;272(11):7085-92.; PMID: 9054401). Primary incubation was 1 hour
IHC: Paraffin embedded Human Skeletal Muscle.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using NBP1-49840 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Glut4

Glucose Transporter 4 (GLUT4/SLC2A4) is a member of a family of proteins that facilitate glucose transport. In response to insulin GLUT4 translocates from low density microsomes to the plasma membrane. Activation of phosphatidylinositol-3-OH kinase is required for this trafficking event but is not sufficient. Insulin resistant subjects with and without diabetes show abnormal accumulation of GLUT4 in a dense membrane compartment in muscle and adipocytes from which insulin is unable to recruit GLUT4 to the cell surface.

Alternate Names

SLC2A4

Entrez Gene IDs

6517 (Human); 20528 (Mouse); 25139 (Rat)

Gene Symbol

SLC2A4

UniProt

P14672

Additional Glut4 Products

Product Documents for Glut4 Antibody

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Certificate of Analysis

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Product Specific Notices for Glut4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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