Glutamate Dehydrogenase Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-54961

Novus Biologicals, part of Bio-Techne
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NBP1-54961
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

View all Glutamate Dehydrogenase 1/GLUD1 Antibodies »

Product Specifications

Immunogen

Synthetic peptides corresponding to GLUD1(glutamate dehydrogenase 1) The peptide sequence was selected from the N terminal of GLUD1. Peptide sequence EGFFDRGASIVEDKLVEDLRTRESEEQKRNRVRGILRIIKPCNHVLSLSF. The peptide sequence for this immunogen was taken from within the described region.

Marker

Mitochondria Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for Glutamate Dehydrogenase Antibody

Western Blot: Glutamate Dehydrogenase Antibody [NBP1-54961]

Western Blot: Glutamate Dehydrogenase Antibody [NBP1-54961]

Western Blot: Glutamate Dehydrogenase Antibody [NBP1-54961] - The results of western blotting and RT-PCR assayA. shows Western blotting results, where the PDHA1 protein expression in the parental LNCaP cells is strongly positive, but its expression in the PDHA1 KO cells is almost negative with two different PDHA1 antibodies. Comparatively, the protein expression of the MPC1 and MPC2 is comparatively decreased and the protein expression of the GLUT1, HK2, LDHA, HIF1alpha, GLS1 and GLUD1 (Glutamate Dehydrogenase) is increased in the LNCaP PDHA1 KO cells. GAPDH was added as a loading control. Image collected and cropped by CiteAb from the following publication (www.oncotarget.com/lookup/doi/10.18632/oncotarget.10782) licensed under a CC-BY license.
Immunohistochemistry: Glutamate Dehydrogenase Antibody [NBP1-54961]

Immunohistochemistry: Glutamate Dehydrogenase Antibody [NBP1-54961]

Immunohistochemistry: Glutamate Dehydrogenase Antibody [NBP1-54961] - GLS1 and GLUD1 (Glutamate Dehydrogenase) are over-expressed in prostate cancers and associated with poor survival. Immunohistochemical staining of GLUD1 in human prostate cancers with Gleason score 4 (D), 7 (E). and 9 (F). Image collected and cropped by CiteAb from the following publication (www.oncotarget.com/lookup/doi/10.18632/oncotarget.10782) licensed under a CC-BY license.
Western Blot: Glutamate Dehydrogenase Antibody [NBP1-54961]

Western Blot: Glutamate Dehydrogenase Antibody [NBP1-54961]

Western Blot: Glutamate Dehydrogenase Antibody [NBP1-54961] - HT1080 cell lysate, concentration 1ug/ml.
View 4 more images

Applications for Glutamate Dehydrogenase Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1.0 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Glutamate Dehydrogenase 1/GLUD1

L-glutamate dehydrogenase (EC 1.4.1.3) has a central role in nitrogen metabolism in plants and animals. Glutamate dehydrogenase is found in all organisms and catalyzes the oxidative deamination of 1-glutamate to 2-oxoglutarate. Glutamate, the main substrate of GLUD, is present in brain in concentrations higher than in other organs. In nervous tissue, GLUD appears to function in both the synthesis and the catabolism of glutamate and perhaps in ammonia detoxification. L-glutamate dehydrogenase (EC 1.4.1.3) has a central role in nitrogen metabolism in plants and animals. Glutamate dehydrogenase is found in all organisms and catalyzes the oxidative deamination of 1-glutamate to 2-oxoglutarate (Smith et al., 2001 [PubMed 11254391]). Glutamate, the main substrate of GLUD, is present in brain in concentrations higher than in other organs. In nervous tissue, GLUD appears to function in both the synthesis and the catabolism of glutamate and perhaps in ammonia detoxification (Mavrothalassitis et al., 1988 [PubMed 3368458]).[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-51 M20867.1 1-51 52-3120 BC112946.1 16-3084

Alternate Names

GDH, GDH1

Gene Symbol

GLUD1

UniProt

P00367 (Human)

Additional Glutamate Dehydrogenase 1/GLUD1 Products

Product Documents for Glutamate Dehydrogenase Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Glutamate Dehydrogenase Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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