Glutamate Dehydrogenase Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-54715
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (96%), Rat (96%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for Glutamate Dehydrogenase Antibody
Immunogen
This antibody was developed against a Recombinant Protein corresponding to amino acids: TFVVQGFGNVGLHSMRYLHRFGAKCIAVGESDGSIWNPDGIDPKELEDFKLQHGSILGFPKAKPYEGSIL
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Glutamate Dehydrogenase Antibody
Western Blot: Glutamate Dehydrogenase Antibody [NBP2-54715]
Western Blot: Glutamate Dehydrogenase Antibody [NBP2-54715] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Human cell line RT-4 Lane 3: Human cell line U-251 MG Lane 4: Human plasma Lane 5: Human Liver tissue Lane 6: Human Tonsil tissueImmunocytochemistry/ Immunofluorescence: Glutamate Dehydrogenase Antibody [NBP2-54715]
Immunocytochemistry/Immunofluorescence: Glutamate Dehydrogenase Antibody [NBP2-54715] - Staining of human cell line A-431 shows localization to mitochondria.Immunohistochemistry-Paraffin: Glutamate Dehydrogenase Antibody [NBP2-54715]
Immunohistochemistry-Paraffin: Glutamate Dehydrogenase Antibody [NBP2-54715] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.Applications for Glutamate Dehydrogenase Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:1000 - 1:2500
Immunohistochemistry-Paraffin
1:1000 - 1:2500
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Glutamate Dehydrogenase 1/GLUD1
Alternate Names
GDH, GDH1
Gene Symbol
GLUD1
Additional Glutamate Dehydrogenase 1/GLUD1 Products
Product Documents for Glutamate Dehydrogenase Antibody
Product Specific Notices for Glutamate Dehydrogenase Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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