Glutaminase Antibody (8R8J6)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15340

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP3-15340-100ul
NBP3-15340-20ul
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 8R8J6

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Glutaminase Antibodies »

Product Summary for Glutaminase Antibody (8R8J6)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Glutaminase (O94925). MMRLRGSGMLRDLLLRSPAGVSATLRRAQPLVTLCRRPRGGGRPAAGPAAAARLHPWWGGGGWPAEPLARGLSSSPSEILQELGKGSTHPQPGVSPPAAP

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Glutaminase Antibody (8R8J6)

Western Blot: Glutaminase Antibody (8R8J6) [NBP3-15340]

Western Blot: Glutaminase Antibody (8R8J6) [NBP3-15340]

Western Blot: Glutaminase Antibody (8R8J6) [NBP3-15340] - Western blot analysis of extracts of various cell lines, using Glutaminase (GLS) Rabbit mAb (NBP3-15340) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Immunocytochemistry/ Immunofluorescence: Glutaminase Antibody (8R8J6) [NBP3-15340]

Immunocytochemistry/ Immunofluorescence: Glutaminase Antibody (8R8J6) [NBP3-15340]

Immunocytochemistry/Immunofluorescence: Glutaminase Antibody (8R8J6) [NBP3-15340] - Immunofluorescence analysis of U-2 OS cells using Glutaminase (GLS) Rabbit mAb (NBP3-15340) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Applications for Glutaminase Antibody (8R8J6)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Glutaminase

Sahai (1983) [PubMed 6825316] demonstrated phosphate-activated glutaminase (EC 3.5.1.2) in human platelets. It is the major enzyme yielding glutamate from glutamine. Significance of the enzyme derives from its possible implication in behavior disturbances in which glutamate acts as a neurotransmitter (Prusiner, 1981). High heritability of platelet glutaminase was indicated by studies of Sahai and Vogel (1983) [PubMed 6682827] who found an intraclass correlation coefficient of 0.96 for monozygotic twins and 0.53 for dizygotic twins.[supplied by OMIM]

Long Name

Glutaminase Kidney Isoform, Mitochondrial

Alternate Names

GLS, K-Glutaminase, L-Glutamine Amidohydrolase

Gene Symbol

GLS

Additional Glutaminase Products

Product Documents for Glutaminase Antibody (8R8J6)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Glutaminase Antibody (8R8J6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.