Glutamine Synthetase Antibody (CL2999)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-46643

Novus Biologicals, part of Bio-Techne
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NBP2-46643
NBP2-46643-25ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL2999

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Glutamine Synthetase Antibodies »

Product Specifications

Immunogen

Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence LQSEGSNSDMYLVPAAMFRDPFRKDPNKLVLCEVFKYNRRPAETNLRHTCKRIMDMVSNQHPWFGMEQEYTLMGTDGHPFGWPSNGFPGPQGPYYCGVGADRAYGRDIVEAHYRACLYAGVKIAGTNAEVMPAQWEFQIGP

Epitope

LCEVFKYNRR

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Glutamine Synthetase Antibody (CL2999)

Western Blot: Glutamine Synthetase Antibody (CL2999) [NBP2-46643]

Western Blot: Glutamine Synthetase Antibody (CL2999) [NBP2-46643]

Western Blot: Glutamine Synthetase Antibody (2999) [NBP2-46643] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissue
Immunohistochemistry: Glutamine Synthetase Antibody (CL2999) [NBP2-46643]

Immunohistochemistry: Glutamine Synthetase Antibody (CL2999) [NBP2-46643]

Immunohistochemistry: Glutamine Synthetase Antibody (2999) [NBP2-46643] - Staining of human pancreas shows absence of immunoreactivity (negative control).
Western Blot: Glutamine Synthetase Antibody (CL2999) [NBP2-46643]

Western Blot: Glutamine Synthetase Antibody (CL2999) [NBP2-46643]

Western Blot: Glutamine Synthetase Antibody (2999) [NBP2-46643] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Cerebral Cortex tissue
View 3 more images

Applications for Glutamine Synthetase Antibody (CL2999)

Application
Recommended Usage

Immunohistochemistry

1:5000 - 1:10000

Immunohistochemistry-Paraffin

1:5000 - 1:10000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Glutamine Synthetase

Glutamine synthetase is part of the glutamine synthetase family. Specifically, Glutamine synthetase catalyzes the condensation of glutamate and ammonia to form glutamine, during the metabolism of nitrogen. Ammonia incorporation in animals occurs through the actions of glutamate dehydrogenase and glutamine synthase. Glutamate plays the central role in mammalian nitrogen flow, serving as both a nitrogen donor and nitrogen acceptor.

Glutamine synthetase antibodies are useful tools for studying nitrogen metabolism and regulation.

Alternate Names

cell proliferation-inducing protein 59, EC 4.1.1.15, EC 6.3.1.2, GLNSPIG59, Glutamate decarboxylase, glutamate-ammonia ligase, Glutamate--ammonia ligase, glutamate-ammonia ligase (glutamine synthase), glutamine synthase, glutamine synthetase, GS, PIG43, proliferation-inducing protein 43

Entrez Gene IDs

2752 (Human)

Gene Symbol

GLUL

UniProt

15104 (Human)

Additional Glutamine Synthetase Products

Product Documents for Glutamine Synthetase Antibody (CL2999)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Glutamine Synthetase Antibody (CL2999)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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