Glutathione Peroxidase 4/GPX4 Antibody (7O4Q9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15362

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15362-100ul
NBP3-15362-20ul
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

ELISA, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 7O4Q9

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Glutathione Peroxidase 4/GPX4 Antibodies »

Product Summary for Glutathione Peroxidase 4/GPX4 Antibody (7O4Q9)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Glutathione Peroxidase 4/GPX4 (P36969). MSLGRLCRLLKPALLCGALAAPGLAGTMCASRDDWRCARSMHEFSAKDIDGHMVNLDKYRGFVCIVTNVASQUGKTEVNYTQLVDLHARYAECGLRILAF

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Glutathione Peroxidase 4/GPX4 Antibody (7O4Q9)

Western Blot: Glutathione Peroxidase 4/GPX4 Antibody (7O4Q9) [NBP3-15362]

Western Blot: Glutathione Peroxidase 4/GPX4 Antibody (7O4Q9) [NBP3-15362]

Western Blot: Glutathione Peroxidase 4/GPX4 Antibody (7O4Q9) [NBP3-15362] - Western blot analysis of extracts of various cell lines, using Glutathione Peroxidase 4/GPX4 Rabbit mAb (NBP3-15362) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Knockdown Validated: Glutathione Peroxidase 4/GPX4 Antibody (7O4Q9) [NBP3-15362]

Knockdown Validated: Glutathione Peroxidase 4/GPX4 Antibody (7O4Q9) [NBP3-15362]

Knockdown Validated: Glutathione Peroxidase 4/GPX4 Antibody (7O4Q9) [NBP3-15362] - Western blot analysis of extracts from normal (control) and Glutathione Peroxidase 4/GPX4 knockout U-87MG cell pools, using Glutathione Peroxidase 4/GPX4 antibody (NBP3-15362) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Applications for Glutathione Peroxidase 4/GPX4 Antibody (7O4Q9)

Application
Recommended Usage

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Glutathione Peroxidase 4/GPX4

Glutathione peroxidase catalyzes the reduction of hydrogen peroxide, organic hydroperoxide, and lipid peroxides by reduced glutathione and functions in the protection of cells against oxidative damage. Human plasma glutathione peroxidase has been shown to be a selenium-containing enzyme and the UGA codon is translated into a selenocysteine. Through alternative splicing and transcription initiation, rat produces proteins that localize to the nucleus, mitochondrion, and cytoplasm. In humans, experimental evidence for alternative splicing exists; alternative transcription initiation and the cleavage sites of the mitochondrial and nuclear transit peptides need to be experimentally verified.

Alternate Names

GPX4, PHGPx, snGPx

Gene Symbol

GPX4

Additional Glutathione Peroxidase 4/GPX4 Products

Product Documents for Glutathione Peroxidase 4/GPX4 Antibody (7O4Q9)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Glutathione Peroxidase 4/GPX4 Antibody (7O4Q9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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