Glutathione Peroxidase 4/GPX4 Antibody (9D4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26545

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals, part of Bio-Techne
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NBP3-26545-50ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunohistochemistry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9D4

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Glutathione Peroxidase 4/GPX4 Antibodies »

Product Summary for Glutathione Peroxidase 4/GPX4 Antibody (9D4)

Immunogen

A synthesized peptide derived from Human Glutathione Peroxidase 4/GPX4 [UniProt P36969]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Glutathione Peroxidase 4/GPX4 Antibody (9D4)

Glutathione Peroxidase 4/GPX4 Antibody (9D4)

Immunohistochemistry: Glutathione Peroxidase 4/GPX4 Antibody (9D4) [NBP3-26545] -

Immunohistochemistry: Glutathione Peroxidase 4/GPX4 Antibody (9D4) [NBP3-26545] - Image of Glutathione Peroxidase 4/GPX4 Antibody (9D4) diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Applications for Glutathione Peroxidase 4/GPX4 Antibody (9D4)

Application
Recommended Usage

Immunohistochemistry

1:50-1:200
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl and 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: Glutathione Peroxidase 4/GPX4

Glutathione peroxidase catalyzes the reduction of hydrogen peroxide, organic hydroperoxide, and lipid peroxides by reduced glutathione and functions in the protection of cells against oxidative damage. Human plasma glutathione peroxidase has been shown to be a selenium-containing enzyme and the UGA codon is translated into a selenocysteine. Through alternative splicing and transcription initiation, rat produces proteins that localize to the nucleus, mitochondrion, and cytoplasm. In humans, experimental evidence for alternative splicing exists; alternative transcription initiation and the cleavage sites of the mitochondrial and nuclear transit peptides need to be experimentally verified.

Alternate Names

GPX4, PHGPx, snGPx

Gene Symbol

GPX4

Additional Glutathione Peroxidase 4/GPX4 Products

Product Documents for Glutathione Peroxidase 4/GPX4 Antibody (9D4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Glutathione Peroxidase 4/GPX4 Antibody (9D4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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