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Glutathione Peroxidase 5 Antibody (3B9)

Novus Biologicals, part of Bio-Techne | Catalog # H00002880-M02

Novus Biologicals, part of Bio-Techne
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H00002880-M02

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunoprecipitation, Sandwich ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3B9

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

GPX5 (NP_001500, 122 a.a. ~ 221 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. VRPGGGFVPSFQLFEKGDVNGEKEQKVFSFLKHSCPHPSEILGTFKSISWDPVKVHDIRWNFEKFLVGPDGIPVMRWSHRATVSSVKTDILAYLKQFKTK

Specificity

GPX5 - glutathione peroxidase 5 (epididymal androgen-related protein) (3B9)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Glutathione Peroxidase 5 Antibody (3B9)

Immunoprecipitation: Glutathione Peroxidase 5 Antibody (3B9) [H00002880-M02]

Immunoprecipitation: Glutathione Peroxidase 5 Antibody (3B9) [H00002880-M02]

Immunoprecipitation: Glutathione Peroxidase 5 Antibody (3B9) [H00002880-M02] - Analysis of GPX5 transfected lysate using anti-GPX5 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with GPX5 MaxPab rabbit polyclonal antibody.
Sandwich ELISA: Glutathione Peroxidase 5 Antibody (3B9) [H00002880-M02] - Detection limit for recombinant GST tagged GPX5 is 1 ng/ml as a capture antibody.

Applications for Glutathione Peroxidase 5 Antibody (3B9)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
It has been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Glutathione Peroxidase 5

Glutathione peroxidase (GPX)/glutathione reductase is part of the hydrogen peroxide scavenging system found within the epididymis in the mammalian male reproductive tract. GPX5 expression is epididymis-specific and the transcript is unique from other GPXs because it contains a deletion resulting in an mRNA that does not contain a selenocysteine (UGA) codon (an unusual amino acid present in other GPXs). This deletion also renders the mRNA incapable of encoding an active GPX isoenzyme. For this reason, GPX5 is selenium-independent and has very little activity towards hydrogen peroxide or organic hydroperoxides. GPX5, which is bound to the acrosome of sperm, may act to protect sperm from premature acrosome reaction in the epididymis. [provided by RefSeq]

Alternate Names

EC 1.11.1, EC 1.11.1.9, EGLP, epididymal androgen-related protein, epididymal secretory glutathione peroxidase, Epididymis-specific glutathione peroxidase-like protein, Glutathione peroxidase 5, glutathione peroxidase 5 (epididymal androgen-related protein), GPx-5, GSHPx-5

Entrez Gene IDs

2880 (Human)

Gene Symbol

GPX5

Additional Glutathione Peroxidase 5 Products

Product Documents for Glutathione Peroxidase 5 Antibody (3B9)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Glutathione Peroxidase 5 Antibody (3B9)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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