Glutathione Reductase Antibody (JE31-42)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32391
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE31-42
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptide within Human Glutathione Reductase aa 473-522 / 522. (Uniprot: P00390)
Localization
Mitochondrion; Cytoplasm.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Glutathione Reductase Antibody (JE31-42)
Western Blot: Glutathione Reductase Antibody (JE31-42) [NBP3-32391]
Western Blot: Glutathione Reductase Antibody (JE31-42) [NBP3-32391] - Western blot analysis of Glutathione Reductase on different lysates with Rabbit anti-Glutathione Reductase antibody (NBP3-32391) at 1/2,000 dilution.Lane 1: HepG2 cell lysate (20 ug/Lane)
Lane 2: A549 cell lysate (20 ug/Lane)
Lane 3: K-562 cell lysate (20 ug/Lane)
Lane 4: Jurkat cell lysate (20 ug/Lane)
Lane 5: Mouse liver tissue lysate (40 ug/Lane)
Lane 6: Mouse brain tissue lysate (40 ug/Lane)
Lane 7: Mouse kidney tissue lysate (40 ug/Lane)
Lane 8: Rat lung tissue lysate (40 ug/Lane)
Lane 9: Rat kidney tissue lysate (40 ug/Lane)
Predicted band size: 56 kDa
Observed band size: 56 kDa
Exposure time: 10 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32391) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Applications for Glutathione Reductase Antibody (JE31-42)
Application
Recommended Usage
Western Blot
1:2000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Glutathione Reductase
Alternate Names
GLUR, GRase, GRD1, GSR, HEL-752
Gene Symbol
GSR
Additional Glutathione Reductase Products
Product Documents for Glutathione Reductase Antibody (JE31-42)
Product Specific Notices for Glutathione Reductase Antibody (JE31-42)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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