Glutathione S-Transferase/GST Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB9499
Key Product Details
Species Reactivity
Multi-Species
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 965427
Product Specifications
Immunogen
E. coli-derived recombinant Glutathione S‑Transferase/GST epitope tag
Accession # P08515
Accession # P08515
Specificity
Detects Glutathione S‑Transferase/GST in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Glutathione S-Transferase/GST Antibody
Detection of Glutathione S‑Transferase/GST by Western Blot.
Western blot shows lysates of GST tagged ISG-15, GST tagged Ubiquitin, GST tagged FLAG®, HEK293T human embryonic kidney cell line,E. colitransfected with GST tagged BRDG1-SH2, andSf21 transfected GST tagged PARK1. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Glutathione S-Transferase/GST Monoclonal Antibody (Catalog # MAB9499) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Glutathione S-Transferase/GST as indicated. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Glutathione S-Transferase/GST Antibody
Application
Recommended Usage
Western Blot
0.25 µg/mL
Sample: GST tagged ISG-15, GST tagged Ubiquitin, GST tagged FLAG®, HEK293T human embryonic kidney cell line, E. coli transfected with GST tagged BRDG1-SH2, and Sf 21 transfected GST tagged PARK1
Sample: GST tagged ISG-15, GST tagged Ubiquitin, GST tagged FLAG®, HEK293T human embryonic kidney cell line, E. coli transfected with GST tagged BRDG1-SH2, and Sf 21 transfected GST tagged PARK1
Reviewed Applications
Read 1 review rated 4 using MAB9499 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Glutathione S-Transferase/GST
Additional Glutathione S-Transferase/GST Products
Product Documents for Glutathione S-Transferase/GST Antibody
Product Specific Notices for Glutathione S-Transferase/GST Antibody
For research use only
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