Glutathione S-Transferase mu 1/GSTM1 Antibody (JE36-67)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32392
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE36-67
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptide within Human Glutathione S-Transferase mu 1/GSTM1 aa 81-130 / 218. (Uniprot: P09488)
Localization
Cytoplasm.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Glutathione S-Transferase mu 1/GSTM1 Antibody (JE36-67)
Western Blot: Glutathione S-Transferase mu 1/GSTM1 Antibody (JE36-67) [NBP3-32392]
Western Blot: Glutathione S-Transferase mu 1/GSTM1 Antibody (JE36-67) [NBP3-32392] - Western blot analysis of Glutathione S-Transferase mu 1/GSTM1 on different lysates with Rabbit anti-Glutathione S-Transferase mu 1/GSTM1 antibody (NBP3-32392) at 1/1,000 dilution.Lane 1: HeLa cell lysate
Lane 2: HCT 116 cell lysate
Lane 3: SH-SY5Y cell lysate
Lysates/proteins at 20 ug/Lane.
Predicted band size: 26 kDa
Observed band size: 26 kDa
Exposure time: 1 minute;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32392) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.
Applications for Glutathione S-Transferase mu 1/GSTM1 Antibody (JE36-67)
Application
Recommended Usage
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Glutathione S-Transferase mu 1/GSTM1
Alternate Names
Glutathione STransferase mu 1, GST1, GSTM1, GTH4, GTM1
Gene Symbol
GSTM1
Additional Glutathione S-Transferase mu 1/GSTM1 Products
Product Documents for Glutathione S-Transferase mu 1/GSTM1 Antibody (JE36-67)
Product Specific Notices for Glutathione S-Transferase mu 1/GSTM1 Antibody (JE36-67)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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