Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 12F2

Format

BSA Free

Concentration

1 mg/ml

View all Glutathione Synthetase Antibodies »

Product Summary for Glutathione Synthetase Antibody (12F2) - BSA Free

Immunogen

Recombinant human Glutathione Synthetase (1-474aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Glutathione Synthetase Antibody (12F2) - BSA Free

Western Blot: Glutathione Synthetase Antibody (12F2)BSA Free [NBP2-59455]

Western Blot: Glutathione Synthetase Antibody (12F2)BSA Free [NBP2-59455]

Western Blot: Glutathione Synthetase Antibody (12F2) [NBP2-59455] - Lane 1: MDA-MB231 cell lysate, Lane 2: WiDr cell lysate, Lane 3: SW480 cell lysate, Lane 4.: A427 cell lysate, Lane 5: MCF7 cell lysate Lane 6: 293T cell lysate, Lane 7: HeLa cell lysate, Lane 8: HT1080 cell lysate Lane 9: U87MG cell lysate, Lane 10: A549 cell lysate
Immunocytochemistry/ Immunofluorescence: Glutathione Synthetase Antibody (12F2) - BSA Free [NBP2-59455]

Immunocytochemistry/ Immunofluorescence: Glutathione Synthetase Antibody (12F2) - BSA Free [NBP2-59455]

Immunocytochemistry/Immunofluorescence: Glutathione Synthetase Antibody (12F2) [NBP2-59455] - Analysis of GSS in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GSS antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). Analysis of GSS in Jurkat cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GSS antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Applications for Glutathione Synthetase Antibody (12F2) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Glutathione Synthetase

Glutathione is important for a variety of biological functions, including protection of cells from oxidative damage byfree radicals, detoxification of xenobiotics, and membrane transport. The protein encoded by this gene functions as ahomodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion ofgamma-L-glutamyl-L-cysteine to glutathione. Defects in this gene are a cause of glutathione synthetase deficiency.(provided by RefSeq)

Alternate Names

EC 6.3.2.3, Glutathione synthase, glutathione synthetase, GSH synthetase, GSHS, GSH-S, MGC14098

Gene Symbol

GSS

Additional Glutathione Synthetase Products

Product Documents for Glutathione Synthetase Antibody (12F2) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Glutathione Synthetase Antibody (12F2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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