Glutathione Synthetase Antibody (OTI1B8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-03351

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, In vivo assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1B8

Concentration

0.51 mg/ml

View all Glutathione Synthetase Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human GSS (NP_000169) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

52.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Glutathione Synthetase Antibody (OTI1B8)

Western Blot: Glutathione Synthetase Antibody (OTI1B8) [NBP2-03351]

Western Blot: Glutathione Synthetase Antibody (OTI1B8) [NBP2-03351]

Western Blot: Glutathione Synthetase Antibody (1B8) [NBP2-03351] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Glutathione Synthetase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Glutathione Synthetase.
Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody (1B8) [NBP2-03351] Staining of paraffin-embedded Human prostate tissue using anti-Glutathione Synthetase mouse monoclonal antibody.
Flow Cytometry: Glutathione Synthetase Antibody (OTI1B8) [NBP2-03351]

Flow Cytometry: Glutathione Synthetase Antibody (OTI1B8) [NBP2-03351]

Flow Cytometry: Glutathione Synthetase Antibody (1B8) [NBP2-03351] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Glutathione Synthetase antibody, and then analyzed by flow cytometry.
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Applications for Glutathione Synthetase Antibody (OTI1B8)

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.51 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Glutathione Synthetase

Glutathione is important for a variety of biological functions, including protection of cells from oxidative damage by free radicals, detoxification of xenobiotics, and membrane transport. The protein encoded by this gene functions as a homodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion of gamma-L-glutamyl-L-cysteine to glutathione. Defects in this gene are a cause of glutathione synthetase deficiency. [provided by RefSeq].

Alternate Names

EC 6.3.2.3, Glutathione synthase, glutathione synthetase, GSH synthetase, GSHS, GSH-S, MGC14098

Entrez Gene IDs

2937 (Human)

Gene Symbol

GSS

Additional Glutathione Synthetase Products

Product Documents for Glutathione Synthetase Antibody (OTI1B8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Glutathione Synthetase Antibody (OTI1B8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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