Glycerol Kinase Antibody (8S4T8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15687

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15687-100ul
NBP3-15687-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 8S4T8

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Glycerol Kinase Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 450-559 of human Glycerol Kinase (P32189). IPVVKPSMPETTALGAAMAAGAAEGVGVWSLEPEDLSAVTMERFEPQINAEESEIRYSTWKKAVMKSMGWVTTQSPESGDPSIFCSLPLGFFIVSSMVMLIGARYISGIP

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Glycerol Kinase Antibody (8S4T8)

Western Blot: Glycerol Kinase Antibody (8S4T8) [NBP3-15687]

Western Blot: Glycerol Kinase Antibody (8S4T8) [NBP3-15687]

Western Blot: Glycerol Kinase Antibody (8S4T8) [NBP3-15687] - Western blot analysis of extracts of various cell lines, using Glycerol Kinase (GK) antibody (NBP3-15687) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry-Paraffin: Glycerol Kinase Antibody (8S4T8) [NBP3-15687]

Immunohistochemistry-Paraffin: Glycerol Kinase Antibody (8S4T8) [NBP3-15687]

Immunohistochemistry-Paraffin: Glycerol Kinase Antibody (8S4T8) [NBP3-15687] - Immunohistochemistry of paraffin-embedded mouse testis using Glycerol Kinase Rabbit mAb (NBP3-15687) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western Blot: Glycerol Kinase Antibody (8S4T8) [NBP3-15687]

Western Blot: Glycerol Kinase Antibody (8S4T8) [NBP3-15687]

Western Blot: Glycerol Kinase Antibody (8S4T8) [NBP3-15687] - Western blot analysis of extracts of various cell lines, using Glycerol Kinase (GK) antibody (NBP3-15687) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
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Applications for Glycerol Kinase Antibody (8S4T8)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Glycerol Kinase

Glycerol kinase catalyzes the formation of glycerol 3 phosphate from ATP and glycerol. Dihydroxyacetone and L glyceraldehyde can also act as acceptors; UTP and, in the case of the yeast enzyme, ITP and GTP can act as donors. It provides a way for glycerol derived from fats or glycerides to enter the glycolytic pathway. NB - Glycerol kinase obtained from Cellulomonas spp has an observed molecular weight of 128kDa.

Alternate Names

GK, GK1, GKD, glpK, Glycerokinase

Gene Symbol

GK

Additional Glycerol Kinase Products

Product Documents for Glycerol Kinase Antibody (8S4T8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Glycerol Kinase Antibody (8S4T8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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