Glycogen Phosphorylase BB/GPBB Antibody

Western Blot: Glycogen Phosphorylase BB/GPBB Antibody [NBP1-32799]

Western Blot: Glycogen Phosphorylase BB/GPBB Antibody [NBP1-32799] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GPBB antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301 was used to detect the primary antibody.

Immunohistochemistry-Paraffin: Glycogen Phosphorylase BB/GPBB Antibody [NBP1-32799]

Immunohistochemistry-Paraffin: Glycogen Phosphorylase BB/GPBB Antibody [NBP1-32799] - U87 xenograft. GPBB antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: Glycogen Phosphorylase BB/GPBB Antibody [NBP1-32799] -

Western Blot: Glycogen Phosphorylase BB/GPBB Antibody [NBP1-32799] - ER alpha & ER beta Involvement in Noradrenergic Regulation of VMN GS & GPbb/GPmm Protein Expression. Micropunch-dissected VMN tissue obtained from groups of female rats (n = 6/group) infused into the VMN with Veh or NE after Veh, MPP, or PHTPP pretreatment was analyzed by Western blot for GS (Panel 5A), F(5, 12) = 8.44, p = .0003; GPbb (Panel 5B), F(5, 12) = 12.90, p < .0001; or GPmm (Panel 5C), F(5, 12) = 16.49, p < .0001 protein content. Data show mean normalized protein optical density (O.D.) values ± SEM. *p < .05; **p < .01; ***p < .001. VMN = ventromedial hypothalamic nucleus; GS = glycogen synthase; GPmm = glycogen phosphorylase-muscle type; GPbb = glycogen phosphorylase-brain type; MPP = 1,3-Bis(4-hydroxyphenyl)-4-methyl-5-[4-(2-piperidinylethoxy)phenol]-1H-pyrazole dihydrochloride; PHTPP = 4-[2-phenyl-5,7-bis(trifluoromethyl)pyrazolo[1,5-a]pyrimidin-3-yl]phenol; NE = norepinephrine. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32233668), licensed under a CC-BY license. Not internally tested by Novus Biologicals.