Glycogen Synthase [p Ser641] Antibody (1D1)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26421
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1D1
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Glycogen Synthase [p Ser641] Antibody (1D1)
Immunogen
A synthesized peptide derived from Human Glycogen Synthase [p Ser641] [UniProt P13807]
Modification
p Ser 641
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Glycogen Synthase [p Ser641] Antibody (1D1)
Western Blot: Glycogen Synthase [p Ser641] Antibody (1D1) [NBP3-26421] -
Western Blot: Glycogen Synthase [p Ser641] Antibody (1D1) [NBP3-26421] - Positive Western Blot detected in Hela whole cell lysate, HepG2 whole cell lysate (treated with Calyculin A or EGF).All lanes: Glycogen Synthase [p Ser641] Antibody at 1.12ug/ml.
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 85 KDa
Observed band size: 85 KDa
Immunocytochemistry/Immunofluorescence: Glycogen Synthase [p Ser641] Antibody (1D1) [NBP3-26421] -
Immunocytochemistry/Immunofluorescence: Glycogen Synthase [p Ser641] Antibody (1D1) [NBP3-26421] - Staining of HepG2 cells with Glycogen Synthase [p Ser641] Antibody (1D1) at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).Applications for Glycogen Synthase [p Ser641] Antibody (1D1)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:20-1:200
Western Blot
1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: Glycogen Synthase
Alternate Names
EC 2.4.1.11, glycogen [starch] synthase, muscle, glycogen synthase 1 (muscle), GYSGSY
Gene Symbol
GYS1
Additional Glycogen Synthase Products
Product Documents for Glycogen Synthase [p Ser641] Antibody (1D1)
Product Specific Notices for Glycogen Synthase [p Ser641] Antibody (1D1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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