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Glycophorin A Antibody (JE32-24)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32393

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-32393

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JE32-24

Concentration

1 mg/ml

Product Summary for Glycophorin A Antibody (JE32-24)

Immunogen

Synthetic peptide within Human Glycophorin A aa 115-150 / 150. (Uniprot: P02724)

Localization

Cell membrane.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

16 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Glycophorin A Antibody (JE32-24)

Glycophorin A Antibody (JE32-24)

Immunohistochemistry: Glycophorin A Antibody (JE32-24) [NBP3-32393]

Immunohistochemistry: Glycophorin A Antibody (JE32-24) [NBP3-32393] - Immunohistochemical analysis of paraffin-embedded human bone marrow tissue with Rabbit anti-Glycophorin A antibody (NBP3-32393) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32393) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Glycophorin A Antibody (JE32-24)

Immunohistochemistry: Glycophorin A Antibody (JE32-24) [NBP3-32393]

Immunohistochemistry: Glycophorin A Antibody (JE32-24) [NBP3-32393] - Immunofluorescence analysis of paraffin-embedded human hodgkin lymphoma tissue labeling Glycophorin A with Rabbit anti-Glycophorin A antibody (NBP3-32393) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32393, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Applications for Glycophorin A Antibody (JE32-24)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

1*TBS (pH7.4), 0.05% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Glycophorin A

Glycophorins A (GPA) and B (GPB) are single pass membrane sialoglycoproteins. GPA is the carrier of blood group M and N specificities, while GPB accounts for S and U specificities. GPA and GPB provide the cells with a large mucin like surface and it has been suggested this provides a barrier to cell fusion, so minimizing aggregation between red blood cells in the circulation.

Alternate Names

CD235a, GPA, GYPA

Gene Symbol

GYPA

Additional Glycophorin A Products

Product Documents for Glycophorin A Antibody (JE32-24)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Glycophorin A Antibody (JE32-24)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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