Glycophorin A Antibody (JE32-24)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32393
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE32-24
Concentration
1 mg/ml
Product Summary for Glycophorin A Antibody (JE32-24)
Immunogen
Synthetic peptide within Human Glycophorin A aa 115-150 / 150. (Uniprot: P02724)
Localization
Cell membrane.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
16 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Glycophorin A Antibody (JE32-24)
Immunohistochemistry: Glycophorin A Antibody (JE32-24) [NBP3-32393]
Immunohistochemistry: Glycophorin A Antibody (JE32-24) [NBP3-32393] - Immunohistochemical analysis of paraffin-embedded human bone marrow tissue with Rabbit anti-Glycophorin A antibody (NBP3-32393) at 1/1,000 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32393) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemistry: Glycophorin A Antibody (JE32-24) [NBP3-32393]
Immunohistochemistry: Glycophorin A Antibody (JE32-24) [NBP3-32393] - Immunofluorescence analysis of paraffin-embedded human hodgkin lymphoma tissue labeling Glycophorin A with Rabbit anti-Glycophorin A antibody (NBP3-32393) at 1/200 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32393, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Applications for Glycophorin A Antibody (JE32-24)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Glycophorin A
Alternate Names
CD235a, GPA, GYPA
Gene Symbol
GYPA
Additional Glycophorin A Products
Product Documents for Glycophorin A Antibody (JE32-24)
Product Specific Notices for Glycophorin A Antibody (JE32-24)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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