Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # SPM599

Concentration

0.2 mg/ml

View all Glycophorin A Antibodies »

Product Summary for Glycophorin A Antibody (SPM599)

Immunogen

Recombinant human Glycophorin A protein (Uniprot: P02724)

Localization

Cell surface

Marker

Erythrocyte Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47719)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Glycophorin A Antibody (SPM599)

Western Blot: Glycophorin A Antibody (SPM599) [NBP2-45024]

Western Blot: Glycophorin A Antibody (SPM599) [NBP2-45024]

Western Blot: Glycophorin A Antibody (SPM599) [NBP2-45024] - Western Blot Analysis of K562 cell lysate using Glycophorin A Monoclonal Antibody (SPM599).
Immunohistochemistry-Paraffin: Glycophorin A Antibody (SPM599) [NBP2-45024]

Immunohistochemistry-Paraffin: Glycophorin A Antibody (SPM599) [NBP2-45024]

Immunohistochemistry-Paraffin: Glycophorin A Antibody (SPM599) [NBP2-45024] - Formalin-fixed, paraffin-embedded human Angiosarcoma stained with Glycophorin A Monoclonal Antibody.
Immunohistochemistry-Paraffin: Glycophorin A Antibody (SPM599) [NBP2-45024]

Immunohistochemistry-Paraffin: Glycophorin A Antibody (SPM599) [NBP2-45024]

Immunohistochemistry-Paraffin: Glycophorin A Antibody (SPM599) [NBP2-45024] - Human Placenta stained with Glycophorin A Monoclonal Antibody (SPM599)

Applications for Glycophorin A Antibody (SPM599)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Glycophorin A

Glycophorins A (GPA) and B (GPB) are single pass membrane sialoglycoproteins. GPA is the carrier of blood group M and N specificities, while GPB accounts for S and U specificities. GPA and GPB provide the cells with a large mucin like surface and it has been suggested this provides a barrier to cell fusion, so minimizing aggregation between red blood cells in the circulation.

Alternate Names

CD235a, GPA, GYPA

Gene Symbol

GYPA

UniProt

P02724

Additional Glycophorin A Products

Product Documents for Glycophorin A Antibody (SPM599)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Glycophorin A Antibody (SPM599)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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