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Glycoprotein V/CD42d Antibody (1C2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-00071

Novus Biologicals, part of Bio-Techne
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NBP2-00071-100ug
NBP2-00071-25ug
Conjugate
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Key Product Details

Species Reactivity

Mouse, Rat

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Armenian Hamster IgG Clone # 1C2

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

The immunogen for this antibody is CD42d (platelet glycoprotein V).

Clonality

Monoclonal

Host

Armenian Hamster

Isotype

IgG

Scientific Data Images for Glycoprotein V/CD42d Antibody (1C2)

Flow Cytometry: Glycoprotein V/CD42d Antibody (1C2) [NBP2-00071]

Flow Cytometry: Glycoprotein V/CD42d Antibody (1C2) [NBP2-00071]

Flow Cytometry: GPV Antibody (1C2) [NBP2-00071] - Staining of mouse platelets with Anti-Mouse CD41 FITC and 0.125 ug of Armenian Hamster IgG Isotype Control Purified (left) or 0.125 ug of Anti-Mouse/Rat CD42d Purified (right) followed by Anti-Armenian Hamster IgG PE . Total events were used for analysi

Applications for Glycoprotein V/CD42d Antibody (1C2)

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunoprecipitation

1:10-1:500
Application Notes
This 1C2 antibody has been tested by flow cytometric analysis of mouse platelets. This can be used at less than or equal to 0.25 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. This 1C2 antibody has also been reported for use in immunoprecipitation, and immunohistology staining of frozen tissue sections. t is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2)

Preservative

0.09% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Glycoprotein V/CD42d

The monoclonal antibody 1C2 recognizes mouse and rat CD42d, a single transmembrane protein also known as platelet glycoprotein V (GPV). CD42d interacts with CD42a, CD42b and CD42c to form the receptor for von willebrand factor and thrombin. This functional complex plays a role in platelet adhesion. Expression of CD42d (unlike other markers such as CD41) is restricted to resting platelets and megakaryocytes and therefore is a good marker for development of megakaryocytes.

Alternate Names

CD42d, GP5, GPV

Gene Symbol

GP5

Additional Glycoprotein V/CD42d Products

Product Documents for Glycoprotein V/CD42d Antibody (1C2)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Glycoprotein V/CD42d Antibody (1C2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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