Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry

Label

Biotin

Antibody Source

Recombinant Monoclonal Mouse IgG1 Clone # 508209

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Glycoprotein V/CD42d Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Glycoprotein V/CD42d
Gln17-Gly523
Accession # P40197

Specificity

Detects human Glycoprotein V/CD42d in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human GPVI is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Glycoprotein V/CD42d Antibody (508209) [Biotin]

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Glycoprotein V/CD42d

GPV (platelet glycoprotein V; designated CD42d) is an 83 kDa type I transmembrane (TM) glycoprotein of the leucine-rich repeat (LRR) family. It is expressed exclusively within the platelet / megakaryocyte lineage, where it noncovalently interacts with other platelet TM LRR proteins, GPIb alpha/beta and GPIX, at a ratio of one GPV to two of each other subunit. The GPI-V-IX complex tethers platelets to von Willebrand factor on the surface of injured endothelial cells. Absence of the complex results in Bernard-Soulier syndrome, a rare bleeding disorder. The human GPV cDNA encodes a 560 amino acid (aa) protein with a 16 aa signal sequence, a 507 aa extracellular domain (ECD) containing 15 LRR, a 21 aa TM sequence, and a short (16 aa) cytoplasmic tail that binds calmodulin in resting, but not activated platelets. The human GPV ECD shares 70%, 71% and 81% aa identity with mouse, rat and equine GPV, respectively. GPV can form soluble fragments of 80 kDa by ADAM10 or ADAM17 cleavage after P507, or 69 kDa by thrombin cleavage after R476. High circulating soluble GPV may be an indicator of platelet activation, but may also be caused by high doses of aspirin. The function of GPV is not entirely clear. Deletion of GPV in mice does not produce any obvious change to surface expression or function of GPIb and GPIX, but surface expression of GPV requires GPIb. Deletion studies also indicate that GPV may play a minor role in collagen adhesion, and may modify platelet aggregation in response to thrombin.

Alternate Names

CD42d, GP5, GPV

Gene Symbol

GP5

Additional Glycoprotein V/CD42d Products

Product Documents for Glycoprotein V/CD42d Antibody (508209) [Biotin]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Glycoprotein V/CD42d Antibody (508209) [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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