Glypican 1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13127

Novus Biologicals, part of Bio-Techne
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NBP3-13127
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Key Product Details

Validated by

Knockout/Knockdown, Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human

Predicted:

Rat (90%), Rhesus Macaque (98%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Glypican 1 Antibodies »

Product Summary for Glypican 1 Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human Glypican 1. The exact sequence is proprietary.

Reactivity Notes

Mouse (89%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

62 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for Glypican 1 Antibody

Western Blot: Glypican 1 Antibody [NBP3-13127]

Western Blot: Glypican 1 Antibody [NBP3-13127]

Western Blot: Glypican 1 Antibody [NBP3-13127] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Glypican 1 antibody (NBP3-13127) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: Glypican 1 Antibody [NBP3-13127]

Immunohistochemistry-Paraffin: Glypican 1 Antibody [NBP3-13127]

Immunohistochemistry-Paraffin: Glypican 1 Antibody [NBP3-13127] - Human pancreas, normal (left) and cancer (right). Glypican 1 antibody (NBP3-13127) diluted at 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: Glypican 1 Antibody [NBP3-13127]

Western Blot: Glypican 1 Antibody [NBP3-13127]

Western Blot: Glypican 1 Antibody [NBP3-13127] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody (NBP3-13127) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
View 1 more images

Applications for Glypican 1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Glypican 1

GPC1(Glypican 1) is a cell surface proteoglycan that contains heparan sulphate. The protein is attached to the cell membrane by a GPI anchor. GPC1 is required for efficient TGF-beta1 signaling in pancreatic cancer cells. Members of the glypican-related integral membrane proteoglycan family (GRIPS) may play a role in the control of cell division and growth regulation.

Alternate Names

GPC1

Gene Symbol

GPC1

Additional Glypican 1 Products

Product Documents for Glypican 1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Glypican 1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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