Glypican 3 Antibody (GPC3/1534R) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-54468
Recombinant Monoclonal Antibody.
![Immunocytochemistry/ Immunofluorescence: Glypican 3 Antibody (GPC3/1534R) - Azide and BSA Free [NBP2-54468]](https://resources.bio-techne.com/images/products/Glypican-3-Antibody-GPC3-1534R-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-54468-img0003.jpg "Immunocytochemistry/ Immunofluorescence: Glypican 3 Antibody (GPC3/1534R) - Azide and BSA Free [NBP2-54468]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Rat
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # GPC3/1534R
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for Glypican 3 Antibody (GPC3/1534R) - Azide and BSA Free
Immunogen
Recombinant human full-length Glypican 3 protein (Uniprot: P51654)
Localization
Cytoplasmic
Specificity
Glypican-3 (GPC3) is a glycosylphospatidyl inositol-anchored membrane protein, which may also be found in a secreted form. Anti-GPC3 has been identified as a useful tumor marker for the diagnosis of hepatocellular carcinoma (HCC), hepatoblastoma, melanoma, testicular germ cell tumors, and Wilms tumor and hepatoblastoma, with a low or undetectable expression in normal adjacent tissue. In patients with thyroid cancer, expression of GPC3 is dramatically enhanced in certain types of cancers: 100% in follicular carcinoma and 70% in papillary carcinoma. Expression of GPC3 in follicular carcinoma is significantly higher than that of follicular adenoma. In contrast, GPC3 is not expressed in anaplastic carcinoma.
Marker
Hepatocellular Carcinoma Marker
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
67 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified by Protein A. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-53142).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Glypican 3 Antibody (GPC3/1534R) - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence: Glypican 3 Antibody (GPC3/1534R) - Azide and BSA Free [NBP2-54468]
Immunocytochemistry/Immunofluorescence: Glypican 3 Antibody (GPC3/1534R) - Azide and BSA Free [NBP2-54468] - Immunofluorescence Analysis of MeOH-fixed HepG2 cells labeling Glypican-3 with followed by Goat anti-rabbit IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)![Immunohistochemistry-Paraffin: Glypican 3 Antibody (GPC3/1534R) - Azide and BSA Free [NBP2-54468]](https://resources.bio-techne.com/images/products/Glypican-3-Antibody-GPC3-1534R-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54468-img0001.jpg "Immunohistochemistry-Paraffin: Glypican 3 Antibody (GPC3/1534R) - Azide and BSA Free [NBP2-54468]")
Immunohistochemistry-Paraffin: Glypican 3 Antibody (GPC3/1534R) - Azide and BSA Free [NBP2-54468]
Immunohistochemistry-Paraffin: Glypican 3 Antibody (GPC3/1534R) - Azide and BSA Free [NBP2-54468] - Formalin-fixed, paraffin-embedded human Fetal Liver stained with Glypican-3 Recombinant Rabbit Monoclonal Antibody (GPC3/1534R)![Flow Cytometry: Glypican 3 Antibody (GPC3/1534R) - Azide and BSA Free [NBP2-54468]](https://resources.bio-techne.com/images/products/Glypican-3-Antibody-GPC3-1534R-Azide-and-BSA-Free-Flow-Cytometry-NBP2-54468-img0002.jpg "Flow Cytometry: Glypican 3 Antibody (GPC3/1534R) - Azide and BSA Free [NBP2-54468]")
Flow Cytometry: Glypican 3 Antibody (GPC3/1534R) - Azide and BSA Free [NBP2-54468]
Flow Cytometry: Glypican 3 Antibody (GPC3/1534R) - Azide and BSA Free [NBP2-54468] - Flow Cytometric Analysis of MeOH-fixed HepG2 cells using Glypican 3 Antibody (GPC3/1534R).followed by Goat anti-rabbit- IgG-CF488 (Blue); Isotype Control (Red).Applications for Glypican 3 Antibody (GPC3/1534R) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5 - 1 ug/million cells
Immunocytochemistry/ Immunofluorescence
0.5 - 1 ug/ml
Immunohistochemistry-Paraffin
0.5 - 1.0 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA buffer, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Glypican 3
Alternate Names
GPC3
Gene Symbol
GPC3
Additional Glypican 3 Products
Product Documents for Glypican 3 Antibody (GPC3/1534R) - Azide and BSA Free
Product Specific Notices for Glypican 3 Antibody (GPC3/1534R) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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