GMEB1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-91949

Novus Biologicals, part of Bio-Techne
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NBP1-91949
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (99%), Rat (98%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all GMEB1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: NVVLMPVSTPKPPKRPRLQRPASTTVLSPSPPVQQPQFTVISPITITPVGQSFSMGNIPVATLSQGSSPVTVHTLPSGPQLFRYATVVSS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GMEB1 Antibody

Western Blot: GMEB1 Antibody [NBP1-91949]

Western Blot: GMEB1 Antibody [NBP1-91949]

Western Blot: GMEB1 Antibody [NBP1-91949] - Analysis in control (vector only transfected HEK293T lysate) and GMEB1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: GMEB1 Antibody [NBP1-91949]

Immunocytochemistry/ Immunofluorescence: GMEB1 Antibody [NBP1-91949]

Immunocytochemistry/Immunofluorescence: GMEB1 Antibody [NBP1-91949] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: GMEB1 Antibody [NBP1-91949]

Immunohistochemistry-Paraffin: GMEB1 Antibody [NBP1-91949]

Immunohistochemistry-Paraffin: GMEB1 Antibody [NBP1-91949] - Staining of human rectum shows strong nuclear positivity in glandular cells.

Applications for GMEB1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GMEB1

GMEB1 is a member of KDWK gene family. The product of this gene associates with GMEB2 protein, and the complex is essential for parvovirus DNA replication. Study of rat homolog implicates the role of this gene in modulation of transactivation by the glucocorticoid receptor bound to glucocorticoid response elements. Two alternative spliced transcript variants encoding different isoforms exist.

Alternate Names

DNA-binding protein p96PIF, glucocorticoid modulatory element binding protein 1, glucocorticoid modulatory element-binding protein 1, GMEB-1, P96PIF, Parvovirus initiation factor p96, PIF p96, PIF96

Gene Symbol

GMEB1

Additional GMEB1 Products

Product Documents for GMEB1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GMEB1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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