GNB5 Antibody (3A3)
Novus Biologicals, part of Bio-Techne | Catalog # H00010681-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Proximity Ligation Assay, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 3A3
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
GNB5 (NP_057278, 1 a.a. ~ 90 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MCDQTFLVNVFGSCDKCFKQRALRPVFKKSQQLSYCSTCAEIMATEGLHENETLASLKSEAESLKGKLEEERAKLHDVELHQVAERVEAL
Reactivity Notes
Human. Other species not tested.
Specificity
GNB5 (3A3)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for GNB5 Antibody (3A3)
![Western Blot: GNB5 Antibody (3A3) [H00010681-M01]](https://resources.bio-techne.com/images/products/GNB5-Antibody-3A3-Western-Blot-H00010681-M01-img0003.jpg "Western Blot: GNB5 Antibody (3A3) [H00010681-M01]")
Western Blot: GNB5 Antibody (3A3) [H00010681-M01]
Western Blot: GNB5 Antibody (3A3) [H00010681-M01] - Analysis of GNB5 expression in transfected 293T cell line by GNB5 monoclonal antibody (M01), clone 3A3.Lane 1: GNB5 transfected lysate(38.8 KDa).Lane 2: Non-transfected lysate.![Proximity Ligation Assay: GNB5 Antibody (3A3) [H00010681-M01]](https://resources.bio-techne.com/images/products/GNB5-Antibody-3A3-Proximity-Ligation-Assay-H00010681-M01-img0001.jpg "Proximity Ligation Assay: GNB5 Antibody (3A3) [H00010681-M01]")
Proximity Ligation Assay: GNB5 Antibody (3A3) [H00010681-M01]
Proximity Ligation Assay: GNB5 Antibody (3A3) [H00010681-M01] - Analysis of protein-protein interactions between GNG7 and GNB5. HeLa cells were stained with anti-GNG7 rabbit purified polyclonal 1:1200 and anti-GNB5 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Sandwich ELISA: GNB5 Antibody (3A3) [H00010681-M01] - Detection limit for recombinant GST tagged GNB5 is 0.1 ng/ml as a capture antibody.
Applications for GNB5 Antibody (3A3)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody Reactive against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: GNB5
Alternate Names
FLJ37457, FLJ43714, G protein, beta subunit 5L, G protein, beta-5 subunit, GB5, Gbeta5, guanine nucleotide binding protein (G protein), beta 5, guanine nucleotide-binding protein subunit beta-5, guanine nucleotide-binding protein, beta subunit 5L, Transducin beta chain 5
Entrez Gene IDs
10681 (Human)
Gene Symbol
GNB5
OMIM
604447 (Human)
UniProt
Additional GNB5 Products
Product Documents for GNB5 Antibody (3A3)
Product Specific Notices for GNB5 Antibody (3A3)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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