GOLGA5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-31862

Novus Biologicals, part of Bio-Techne
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NBP1-31862
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Hamster

Predicted:

Bovine (90%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all GOLGA5 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human GOLGA5. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (88%).

Localization

Membrane

Marker

Golgi Apparatus Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

83 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GOLGA5 Antibody

Western Blot: GOLGA5 Antibody [NBP1-31862]

Western Blot: GOLGA5 Antibody [NBP1-31862]

Western Blot: GOLGA5 Antibody [NBP1-31862] - Sample (30 ug of whole cell lysate) A: A549 7. 5% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: GOLGA5 Antibody [NBP1-31862]

Immunocytochemistry/ Immunofluorescence: GOLGA5 Antibody [NBP1-31862]

Immunocytochemistry/Immunofluorescence: GOLGA5 Antibody [NBP1-31862] - Analysis of methanol-fixed HeLa, using GOLGA5 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2500.
Immunohistochemistry-Paraffin: GOLGA5 Antibody [NBP1-31862]

Immunohistochemistry-Paraffin: GOLGA5 Antibody [NBP1-31862]

Immunohistochemistry-Paraffin: GOLGA5 Antibody [NBP1-31862] - Paraffin-embedded smooth muscle, using antibody at 1:100 dilution.
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Applications for GOLGA5 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: GOLGA5

The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes a member of the golgin family of proteins, whose members localize to the Golgi. This protein is a coiled-coil membrane protein that has been postulated to play a role in vesicle tethering and docking. Translocations involving this gene and the ret proto-oncogene have been found in tumor tissues; the chimeric sequences have been designated RET-II and PTC5.

Alternate Names

Cell proliferation-inducing gene 31 protein, golgi autoantigen, golgin subfamily a, 5, golgin A5, Golgin subfamily A member 5, Golgin-84, GOLIM5, Protein Ret-II, PTC5, RET-fused gene 5 protein, RETII, ret-II, rfg5, RFG5golgi integral membrane protein 5

Gene Symbol

GOLGA5

Additional GOLGA5 Products

Product Documents for GOLGA5 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for GOLGA5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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