GOLGA6 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-2436

Novus Biologicals, part of Bio-Techne
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NB100-2436
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all GOLGA6 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-DLPKEKADGTEQ corresponding to internal region according to NP_061122.4, NP_001033729.1.

Specificity

GeneID 55889 may be a pseudogene, while GeneID 342096 is thought to encode a functional protein.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for GOLGA6 Antibody

Immunohistochemistry-Paraffin: GOLGA6 Antibody [NB100-2436]

Immunohistochemistry-Paraffin: GOLGA6 Antibody [NB100-2436]

Immunohistochemistry-Paraffin: GOLGA6 Antibody [NB100-2436] - Paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for GOLGA6 Antibody

Application
Recommended Usage

Immunohistochemistry

3 - 6 ug/ml

Immunohistochemistry-Paraffin

3 - 6 ug/ml

Peptide ELISA

Detection limit 1:1000

Western Blot

1:100 - 1:2000
Application Notes
WB: Preliminary experiments gave an approx. 45 kDa band in human liver lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 79.9 kDa band according to NP_001033729.1. The 45 kDa band was successfully blocked by incubation with the immunizing peptide. IHC-P: Human cerebral cortex shows localized cytoplasm staining consistent with Golgi.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GOLGA6

GOLGA6 is a member of the golgin family of proteins, whose members localise to the Golgi appartus. This gene is found in a large, low copy repeat sequence or duplicon that is found in multiple copies, that are greater than 90% similar, on chromosome 15. Duplicons are associated with deletions, inversions and other chromosome rearrangements that underlie genomic disease. The protein encoded by this gene is thought to be a functional golgin protein while the majority of the related copies of this gene are thought to be transcribed pseudogenes.

Alternate Names

FLJ75859, GLPGolgin-like protein, GOLGA6Golgin linked to PML, golgi autoantigen, golgin subfamily a, 6, golgi autoantigen, golgin subfamily a, 6A, golgi autoantigen, golgin subfamily a, member 6, golgin A6 family, member A, Golgin subfamily A member 6A

Entrez Gene IDs

342096 (Human)

Gene Symbol

GOLGA6A

UniProt

NC_000015.8 (Human)

Additional GOLGA6 Products

Product Documents for GOLGA6 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GOLGA6 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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