Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all GOLGB1/Giantin Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 425 to 475 of human Golgin B1 using the numbering given in entry NP_004478.3 (GeneID 2804).

Marker

Golgi Apparatus Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GOLGB1/Giantin Antibody

Western Blot: GOLGB1/Giantin Antibody [NBP2-22321]

Western Blot: GOLGB1/Giantin Antibody [NBP2-22321]

Western Blot: GOLGB1/Giantin Antibody [NBP2-22321] - HeLa cell lysate sample treated with DMSO was run on 8% SDS-PAGE. Both giantin monomer and dimer are indicated. Western blot image submitted by a verified customer review.
Immunocytochemistry/ Immunofluorescence: GOLGB1/Giantin Antibody [NBP2-22321]

Immunocytochemistry/ Immunofluorescence: GOLGB1/Giantin Antibody [NBP2-22321]

Immunocytochemistry/Immunofluorescence: GOLGB1/Giantin Antibody [NBP2-22321] - PC-3 human prostate cancer cell line. Image was captured using EVOS M5000 microscope. Red - giantin; blue - nucleus, DAPI; bar 40 micron. ICC/IF image submitted by a verified customer review.
Western Blot: GOLGB1/Giantin Antibody [NBP2-22321]

Western Blot: GOLGB1/Giantin Antibody [NBP2-22321]

Western Blot: GOLGB1/Giantin Antibody [NBP2-22321] - Whole cell lysate (50 ug) from 293T, HeLa, and Jurkat cells. NBP2-22321 used for WB at 0.1 ug/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.
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Applications for GOLGB1/Giantin Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Validated from a verified customer review.

Immunoprecipitation

2 - 10 ug/mg

Western Blot

1:2000 - 1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 5 using NBP2-22321 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: GOLGB1/Giantin

The giantin (GOLGB1) gene codes for a golgin subfamily B member 1 protein that exists in isoform 1 at a length of 3,259 amino acids and a mass of 376 kDa or isoform 2 at 3,269 amino acids long and 377 kDA. GOLGB1 is thought to participate in the development of intercisternal cross-bridges of the Golgi complex. Researchers have investigated its role in hypertension, myopathy, hematopoiesis, encephalitis, and arthritis. GOLGB1 is known to interact with other genes such as PFN2, CAV2, USO1, SLC2A3, and GORASP2.

Long Name

Golgin B1

Alternate Names

GCP372, Giantin, GOLIM1, Macrogolgin

Gene Symbol

GOLGB1

UniProt

NP_004478.3 (Human)

Additional GOLGB1/Giantin Products

Product Documents for GOLGB1/Giantin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for GOLGB1/Giantin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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