Golgi Glycoprotein 1/GLG1 Antibody (GLG1/970) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47794
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse (Negative), Rat (Negative)
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # GLG1/970
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Golgi fraction from human liver cells
Reactivity Notes
Does not react with Mouse or Rat.
Localization
Golgi complex in cytoplasm
Specificity
This monoclonal antibody recognizes a protein of 134kDa, which binds fibroblast growth factor and E-selectin (cell-adhesion lectin on endothelial cells mediating the binding of neutrophils). Fucosylation is essential for binding to E-selectin. It contains sialic acid residues and 16 Cys-rich GLG1 repeats. This monoclonal antibody can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells. This monoclonal antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. The Golgi apparatus is an organelle present in all eukaryotic cells that forms a part of the endomembrane system. The primary function of the Golgi apparatus is to process and package macromolecules synthesized by the cell for exocytosis or use within the cell. The Golgi is made up of a stack of flattened, membrane-bound sacs known as cisternae, with three functional regions: the cis face, medial region and trans face. Each region consists of various enzymes that selectively modify the macromolecules passing though them, depending on where they are destined to reside. Several spherical vesicles that have budded off of the Golgi are present surrounding the main cisternae. The Golgi tends to be more pronounced and numerous in cells that make and secrete many substances such as plasma B cells.
Marker
Marker for Human Cells
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
134 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA. Also available at 200 ug/ml WITH BSA & azide (NBP2-44361).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Golgi Glycoprotein 1/GLG1 Antibody (GLG1/970) - Azide and BSA Free
Immunohistochemistry-Paraffin: Golgi Glycoprotein 1/GLG1 Antibody (GLG1/970) - Azide and BSA Free [NBP2-47794]
Immunohistochemistry-Paraffin: Golgi Glycoprotein 1/GLG1 Antibody (GLG1/970) - Azide and BSA Free [NBP2-47794] - Human Pancreas stained with GLG1 Monoclonal Antibody (GLG1/970).Applications for Golgi Glycoprotein 1/GLG1 Antibody (GLG1/970) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunohistochemistry (Formalin-fixed): 0.1-0.2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Golgi Glycoprotein 1/GLG1
Alternate Names
CFR-1, ESL-1, GLG1, MG160
Gene Symbol
GLG1
Additional Golgi Glycoprotein 1/GLG1 Products
Product Documents for Golgi Glycoprotein 1/GLG1 Antibody (GLG1/970) - Azide and BSA Free
Product Specific Notices for Golgi Glycoprotein 1/GLG1 Antibody (GLG1/970) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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