Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all GOLPH3 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 14 amino acid synthetic peptide from near the amino terminus of human GOLPH3. The immunogen is located within the first 50 amino acids of GOLPH3. Amino Acid Squence: SEDDAQSRRDEQDD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GOLPH3 Antibody - BSA Free

Western Blot: GOLPH3 AntibodyBSA Free [NBP1-76241]

Western Blot: GOLPH3 AntibodyBSA Free [NBP1-76241]

Western Blot: GOLPH3 Antibody [NBP1-76241] - Western blot analysis of GOLPH3 in rat lung tissue lysate with GOLPH3 antibody at (A) 0.5 and (B) 1 ug/mL.
GOLPH3 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: GOLPH3 Antibody - BSA Free [NBP1-76241] -

Immunocytochemistry/ Immunofluorescence: GOLPH3 Antibody - BSA Free [NBP1-76241] - Immunofluorescence of GOLPH3 in Rat Lung cells with GOLPH3 antibody at 20 u/mL.

Applications for GOLPH3 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/ml

Western Blot

0.5-1 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GOLPH3

GOLPH3 was initially identified as a peripheral membrane protein localized to the trans-Golgi network, but others reported it to be primarily a mitochondrial protein that regulated the mitochondrial mass through the regulation of the mitochondria-specific phospholipid cardiolipin. GOLPH3 has since been implicated in the target of rapamycin (TOR) signalling pathway. Its overexpression in transfected cells led to and increase in anchorage-independent growth and cell proliferation in vitro. Furthermore, GOLPH3-transfected cells enhanced S6 Kinase activity in response to growth factor stimulation by EGF. Simultaneously, AKT phosphorylation increased in these cells, while these events were abrogated in GOLPH3 siRNA treated cells compared to control cells, indicating the GOLPH3 can enhance signalling through TOR-associated complexes. These results suggest that GOLPH3 is a bona fide oncogene and may be a useful target for therapeutic strategies.

Alternate Names

Coat protein GPP34, FLJ90675, Golgi phosphoprotein 3, golgi phosphoprotein 3 (coat-protein), golgi protein, golgi-associated protein, GOPP1, GPP34coat-protein, MIDASgolgi peripheral membrane protein 1, 34 kDa, Mitochondrial DNA absence factor

Entrez Gene IDs

64083 (Human)

Gene Symbol

GOLPH3

UniProt

Q9H4A6 (Human)

Additional GOLPH3 Products

Product Documents for GOLPH3 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for GOLPH3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.