Goosecoid Antibody (OTI1D7)
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-47790
![Western Blot: Goosecoid Antibody (OTI1D7) [NBP1-47790]](https://resources.bio-techne.com/images/products/Goosecoid-Antibody-1D7-Western-Blot-NBP1-47790-img0015.jpg "Western Blot: Goosecoid Antibody (OTI1D7) [NBP1-47790]")
![Western Blot: Goosecoid Antibody (OTI1D7) [NBP1-47790]](https://resources.bio-techne.com/images/products/Goosecoid-Antibody-1D7-Western-Blot-NBP1-47790-img0016.jpg "Western Blot: Goosecoid Antibody (OTI1D7) [NBP1-47790]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI1D7
Concentration
0.7 mg/ml
Product Summary for Goosecoid Antibody (OTI1D7)
Immunogen
Human recombinant protein fragment corresponding to amino acids 107-257 of human GSC (NP_776248) produced in E.coli.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Specificity
This antibody is specific for Homo sapiens goosecoid homeobox (GSC).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Goosecoid Antibody (OTI1D7)
Western Blot: Goosecoid Antibody (OTI1D7) [NBP1-47790]
Western Blot: Goosecoid Antibody (1D7) [NBP1-47790] - Analysis of extracts (35ug) from 9 different cell lines by using anti-GSC monoclonal antibody.![Immunohistochemistry-Paraffin: Goosecoid Antibody (OTI1D7) [NBP1-47790]](https://resources.bio-techne.com/images/products/Goosecoid-Antibody-1D7-Immunohistochemistry-Paraffin-NBP1-47790-img0014.jpg "Immunohistochemistry-Paraffin: Goosecoid Antibody (OTI1D7) [NBP1-47790]")
Immunohistochemistry-Paraffin: Goosecoid Antibody (OTI1D7) [NBP1-47790]
Immunohistochemistry-Paraffin: Goosecoid Antibody (1D7) [NBP1-47790] - Staining of paraffin-embedded Human prostate tissue using anti-GSC mouse monoclonal antibody.![Flow Cytometry: Goosecoid Antibody (OTI1D7) [NBP1-47790]](https://resources.bio-techne.com/images/products/Goosecoid-Antibody-1D7-Flow-Cytometry-NBP1-47790-img0009.jpg "Flow Cytometry: Goosecoid Antibody (OTI1D7) [NBP1-47790]")
Flow Cytometry: Goosecoid Antibody (OTI1D7) [NBP1-47790]
Flow Cytometry: Goosecoid Antibody (1D7) [NBP1-47790] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-GSC antibody, and then analyzed by flow cytometry.![Immunohistochemistry-Paraffin: Goosecoid Antibody (OTI1D7) [NBP1-47790]](https://resources.bio-techne.com/images/products/Goosecoid-Antibody-1D7-Immunohistochemistry-Paraffin-NBP1-47790-img0010.jpg "Immunohistochemistry-Paraffin: Goosecoid Antibody (OTI1D7) [NBP1-47790]")
![Immunohistochemistry-Paraffin: Goosecoid Antibody (OTI1D7) [NBP1-47790]](https://resources.bio-techne.com/images/products/Goosecoid-Antibody-1D7-Immunohistochemistry-Paraffin-NBP1-47790-img0011.jpg "Immunohistochemistry-Paraffin: Goosecoid Antibody (OTI1D7) [NBP1-47790]")
![Immunohistochemistry-Paraffin: Goosecoid Antibody (OTI1D7) [NBP1-47790]](https://resources.bio-techne.com/images/products/Goosecoid-Antibody-1D7-Immunohistochemistry-Paraffin-NBP1-47790-img0012.jpg "Immunohistochemistry-Paraffin: Goosecoid Antibody (OTI1D7) [NBP1-47790]")
![Immunohistochemistry-Paraffin: Goosecoid Antibody (OTI1D7) [NBP1-47790]](https://resources.bio-techne.com/images/products/Goosecoid-Antibody-1D7-Immunohistochemistry-Paraffin-NBP1-47790-img0013.jpg "Immunohistochemistry-Paraffin: Goosecoid Antibody (OTI1D7) [NBP1-47790]")
Applications for Goosecoid Antibody (OTI1D7)
Application
Recommended Usage
Flow Cytometry
1:100
Immunohistochemistry
1:50
Immunohistochemistry-Paraffin
1:50
Western Blot
1:500-1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.7 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Goosecoid
Additional Goosecoid Products
Product Documents for Goosecoid Antibody (OTI1D7)
Product Specific Notices for Goosecoid Antibody (OTI1D7)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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