GP2 Antibody (GP2/1712) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-54416
![Western Blot: GP2 Antibody (GP2/1712)Azide and BSA Free [NBP2-54416]](https://resources.bio-techne.com/images/products/GP2-Antibody-GP2-1712-Azide-and-BSA-Free-Western-Blot-NBP2-54416-img0003.jpg "Western Blot: GP2 Antibody (GP2/1712)Azide and BSA Free [NBP2-54416]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse (Negative)
Applications
CyTOF-ready, ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Lambda Clone # GP2/1712
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant fragment of human GP2 protein (around aa 35-179) (exact sequence is proprietary) (Uniprot: P55259)
Reactivity Notes
Does not react with Mouse.
Localization
Cell Surface and Secreted
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Lambda
Theoretical MW
59 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-53322).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for GP2 Antibody (GP2/1712) - Azide and BSA Free
Western Blot: GP2 Antibody (GP2/1712)Azide and BSA Free [NBP2-54416]
Western Blot: GP2 Antibody (GP2/1712) - Azide and BSA Free [NBP2-54416] - Western Blot Analysis of human Pancreas tissue lysate using GP2 antibody (GP2/1712).![Immunohistochemistry-Paraffin: GP2 Antibody (GP2/1712) - Azide and BSA Free [NBP2-54416]](https://resources.bio-techne.com/images/products/GP2-Antibody-GP2-1712-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54416-img0001.jpg "Immunohistochemistry-Paraffin: GP2 Antibody (GP2/1712) - Azide and BSA Free [NBP2-54416]")
Immunohistochemistry-Paraffin: GP2 Antibody (GP2/1712) - Azide and BSA Free [NBP2-54416]
Immunohistochemistry-Paraffin: GP2 Antibody (GP2/1712) - Azide and BSA Free [NBP2-54416] - Formalin-fixed, paraffin-embedded human Pancreas stained with GP2 Monoclonal Antibody (GP2/1712).![Protein Array: GP2 Antibody (GP2/1712) - Azide and BSA Free [NBP2-54416]](https://resources.bio-techne.com/images/products/GP2-Antibody-GP2-1712-Azide-and-BSA-Free-Protein-Array-NBP2-54416-img0002.jpg "Protein Array: GP2 Antibody (GP2/1712) - Azide and BSA Free [NBP2-54416]")
Protein Array: GP2 Antibody (GP2/1712) - Azide and BSA Free [NBP2-54416]
Protein Array: GP2 Antibody (GP2/1712) - Azide and BSA Free [NBP2-54416] - Analysis of Protein Array containing more than 19,000 full-length human proteins using GP2 Antibody (GP2/1712) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5.Applications for GP2 Antibody (GP2/1712) - Azide and BSA Free
Application
Recommended Usage
ELISA
2-4 ug/ml
Immunohistochemistry-Paraffin
1 - 2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
ELISA: Use Ab at 2-4ug/ml for coating. Order Ab without BSA.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: GP2/ZAP75
Long Name
Glycoprotein 2
Alternate Names
GP2, ZAP75
Gene Symbol
GP2
Additional GP2/ZAP75 Products
Product Documents for GP2 Antibody (GP2/1712) - Azide and BSA Free
Product Specific Notices for GP2 Antibody (GP2/1712) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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