GPAM Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-76907

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Predicted:

Bovine (93%), Porcine (100%). Backed by our 100% Guarantee.

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all GPAM Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of the human GPAT1. The immunogen is located within amino acids 730 - 780 of GPAT1. Amino Acid Squence: SGPVPEPEYLQKLHK

Specificity

At least four isoforms of GPAT1 are known to exist; this antibody will detect only the two largest isoforms.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

92 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GPAM Antibody - BSA Free

Western Blot: GPAM AntibodyBSA Free [NBP1-76907]

Western Blot: GPAM AntibodyBSA Free [NBP1-76907]

Western Blot: GPAM Antibody [NBP1-76907] - Western blot analysis of GPAT1 in rat brain tissue lysate with GPAT1 antibody at (A) 1 and (B) 2 ug/mL.
Immunocytochemistry/ Immunofluorescence: GPAM Antibody - BSA Free [NBP1-76907]

Immunocytochemistry/ Immunofluorescence: GPAM Antibody - BSA Free [NBP1-76907]

Immunocytochemistry/Immunofluorescence: GPAM Antibody [NBP1-76907] - Immunofluorescence of GPAT1 in Rat Brain tissue with GPAT1 antibody at 20 ug/mL.
Immunohistochemistry-Paraffin: GPAM Antibody - BSA Free [NBP1-76907]

Immunohistochemistry-Paraffin: GPAM Antibody - BSA Free [NBP1-76907]

Immunohistochemistry-Paraffin: GPAM Antibody [NBP1-76907] - Staining of GPAT1 in mouse brain tissue with GPAT1 antibody at 5 ug/ml.
View 8 more images

Applications for GPAM Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/ml

Immunohistochemistry

2.5 ug/mL

Western Blot

1-2 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GPAM

Glycerol-3-phosphate acyltransferase 1 (GPAT1), one of four known GPAT isoforms, is located on the mitochondrial outer membrane, allowing reciprocal regulation with carnitine palmitoyltransferase-1. It is thought to be critical for the development of hepatic steatosis; steatosis triggered by GPAT1 overexpression leads to hepatic and possibly peripheral insulin resistance. GPAT1 is transcriptionally upregulated by insulin and sterol regulatory element binding protein (SREBP-1) and downregulated by AMP-activated protein kinase. Mice deficient in GPAT1 exhibit decreased triacylglycerol (TAG) in cardiomyocytes even in high-fat diets, suggesting that GPAT1 contributes significantly to TAG accumulation in heart tissue during lipogenic or high fat diets. At least two isoforms of GPAT1 are known to exist.

Alternate Names

EC 2.3.1, EC 2.3.1.15, glycerol-3-phosphate acyltransferase 1, mitochondrial, glycerol-3-phosphate acyltransferase, mitochondrial, GPAT, GPAT-1, GPAT1mitochondrial, MGC26846

Gene Symbol

GPAM

UniProt

NP_065969 (Human)

Additional GPAM Products

Product Documents for GPAM Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for GPAM Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.