GPD2 Antibody (2C11-1F4)

Novus Biologicals, part of Bio-Techne | Catalog # H00002820-M02

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2C11-1F4

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all GPD2 Antibodies »

Product Specifications

Immunogen

GPD2 (AAH19874, 1 a.a. ~ 378 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAFQKAVKGTLVGGGALATVLGLSQFAHYRRKQMNLAYVKAADCISEPVNREPPSREAQLLTLQNTSEFDILVIGGGATGSGCALDAVTRGLKTALVERDDFSSGTSSRSTKLIHGGVRYLQKAIMKLDIEQYRMVKEALHERANLLEIAPHLSAPLPIMLPVYKWWQLPYYWVGIKLYDLVAGSNCLKSSYVLSKSRALEHFPMLQKDKLVGAIVYYDGQHNDARMNLAIALTAARYGAATANYMEVVSLLKKTDPQTGKVHVSGARCKDVLTGQEFDVRAKCVINATGPFTDSVRKMDDKDAAAICQPSAGVHIVMPGYYSPESMGLLDPATSDGRVIFFLPWQKMTIAGTTDTPTDVTHHPIPSEEDINFILNEV

Specificity

GPD2 - glycerol-3-phosphate dehydrogenase 2 (mitochondrial)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for GPD2 Antibody (2C11-1F4)

Western Blot: GPD2 Antibody (2C11-1F4) [H00002820-M02]

Western Blot: GPD2 Antibody (2C11-1F4) [H00002820-M02]

Western Blot: GPD2 Antibody (2C11-1F4) [H00002820-M02] - Western Blot analysis of GPD2 expression in transfected 293T cell line by GPD2 monoclonal antibody (M02), clone 2C11-1F4. Lane 1: GPD2 transfected lysate (41 KDa). Lane 2: Non-transfected lysate.
Sandwich ELISA: GPD2 Antibody (2C11-1F4) [H00002820-M02] - Detection limit for recombinant GST tagged GPD2 is approximately 1ng/ml as a capture antibody.

Applications for GPD2 Antibody (2C11-1F4)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: GPD2

Mitochondrial glycerophosphate dehydrogenase (EC 1.1.99.5), or GPD2, is located on the outer surface of the inner mitochondrial membrane and catalyzes the unidirectional conversion of glycerol-3-phosphate (G-3-P) to dihydroxyacetone phosphate (DHAP) with concomitant reduction of the enzyme-bound FAD. Together with a cytosolic NAD-linked GPD (GPD1; MIM 138420), GPD2 forms the glycerol phosphate shuttle, which uses the interconversion of G-3-P and DHAP to transfer reducing equivalents into mitochondria, resulting in the reoxidation of NADH formed during glycolysis.[supplied by OMIM]

Long Name

Glycerol-3-phosphate dehydrogenase, mitochondrial

Alternate Names

EC 1.1.5.3, GPD-M, GPDH-M, mGDH, mtGPD

Entrez Gene IDs

2820 (Human)

Gene Symbol

GPD2

OMIM

125 (Human)

UniProt

AAH19874 (Human)

Additional GPD2 Products

Product Documents for GPD2 Antibody (2C11-1F4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GPD2 Antibody (2C11-1F4)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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