GPER/GPR30 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NLS1183

Novus Biologicals, part of Bio-Techne
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NLS1183
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Predicted:

Primate (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all GPER/GPR30 Antibodies »

Product Specifications

Immunogen

Synthetic 16 amino acid peptide from 1st cytoplasmic domain of human GPER/GPR30.

Epitope

1st cytoplasmic domain

Reactivity Notes

Mouse reactivity reported in scientific literature (Natale C et al). Predicted cross-reactivity based on sequence identity: Gorilla (100%), Marmoset (94%), Monkey (88%).

Localization

Endoplasmic reticulum - multi-pass membrane protein.

Specificity

Human GPR30. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Product can be stored undiluted at 4C for up to 1 month.

Scientific Data Images for GPER/GPR30 Antibody - BSA Free

Immunohistochemistry-Paraffin: GPER/GPR30 Antibody - BSA Free [NLS1183]

Immunohistochemistry-Paraffin: GPER/GPR30 Antibody - BSA Free [NLS1183]

Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NLS1183] - Human breast carcinoma.Tissue after heat-induced antigen retrieval.
Immunohistochemistry-Paraffin: GPER/GPR30 Antibody - BSA Free [NLS1183]

Immunohistochemistry-Paraffin: GPER/GPR30 Antibody - BSA Free [NLS1183]

Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NLS1183] - Analysis of anti-GPER1 / GPR30 antibody with breast carcinoma.
Immunohistochemistry-Paraffin: GPER/GPR30 Antibody - BSA Free [NLS1183]

Immunohistochemistry-Paraffin: GPER/GPR30 Antibody - BSA Free [NLS1183]

Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NLS1183] - Analysis of anti-GPER1 / GPR30 antibody with human, pancreas, islet.
View 6 more images

Applications for GPER/GPR30 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

15 - 20 ug/ml

Western Blot

1:1000 - 1:10000
Application Notes
ICC/IF usage is reported in scientific literature (PMID: 24011568). Use in IHC reported in scientific literature (PMID: 24011568)..
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 4 using NLS1183 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.

Background: GPER/GPR30

GPR30 a former orphan receptor has been linked to estrogen-mediated activation of adenylyl cyclase, release of epidermal growth factor (EGF)-related ligands, and specific estrogen binding.1 GPR30 acts independently of estrogen receptors, ERalpha and ERbeta, to release of heparin-binding EGF and may play a role in breast cancer tumorogenesis.

Long Name

G Protein-coupled Estrogen Receptor 1

Alternate Names

CEPR, CMKRL2, DRY12, FEG-1, GPCR-BR, GPR30, LERGU2, LyGPR

Entrez Gene IDs

2852 (Human)

Gene Symbol

GPER1

UniProt

Q99527

Additional GPER/GPR30 Products

Product Documents for GPER/GPR30 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GPER/GPR30 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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