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GPR116 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14343

Novus Biologicals, part of Bio-Techne
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NBP3-14343

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Primate (100%). Backed by our 100% Guarantee.

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit

Concentration

1 mg/ml

Product Specifications

Immunogen

Synthetic 20 amino acid peptide from internal region of human GPR116.

Epitope

Internal

Specificity

Human GPR116. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Clonality

Polyclonal

Host

Rabbit

Description

Product can be stored undiluted at 4C for up to 1 month.

Scientific Data Images for GPR116 Antibody

Immunohistochemistry-Paraffin: GPR116 Antibody [NBP3-14343]

Immunohistochemistry-Paraffin: GPR116 Antibody [NBP3-14343]

Immunohistochemistry-Paraffin: GPR116 Antibody [NBP3-14343] - GPR116 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Applications for GPR116 Antibody

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after proteinase K antigen retrieval. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 10 ug/ml..

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Preservative

0.1% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.

Background: GPR116

GPR116/KIAA0758 is an Orphan-B GPCR with an unknown ligand. GPR116 has two immunoglobulin-like repeats and a SEA box. RT-PCR analysis from the KIAA database suggests that KIAA0758 is expressed in heart, kidney, lung and ovary. ESTs have been isolated from brain, breast, eye, heart, kidney, liver, liver/spleen, lung, nasopharyngeal, spleen, and testis libraries.

Long Name

G Protein-coupled Receptor 116

Alternate Names

FLJ90640, G protein-coupled receptor 116, Ig-Hepta homolog, KIAA0758DKFZp564O1923, KPG_001, probable G-protein coupled receptor 116

Gene Symbol

ADGRF5

Additional GPR116 Products

Product Documents for GPR116 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GPR116 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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